健脾益气活血汤对自发性高血压大鼠血管内皮舒缩因子表达的影响  

Influence of Jianpi Yiqi Huoxue Decoction on Vasomotor Factors Expression in Vascular Endothelium of Spontaneously Hypertensive Rats

作　　者：曲怡[1] 张立德[1] 陈志娟[1] 王珊珊[1] 谷丽艳[1] 林庶茹[1] 

机构地区：[1]辽宁中医药大学基础医学院中医分子生物学实验室,沈阳110847

出　　处：《中国实验方剂学杂志》2013年第8期221-224,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省科技厅自然科学基金计划项目(20102150)

摘　　要：目的:观察健脾益气活血汤对自发性高血压大鼠(SHR)收缩压及血管内皮舒缩因子表达的影响。方法:采用随机对照方法,将60只24周龄SHR分为SHR组(给予蒸馏水)、培哚普利组(培哚普利,0.4 mg·kg-1.d-1)、培哚普利联合中药组(培哚普利0.4 mg·kg-1.d-1+中药健脾益气活血汤20 g·kg-1.d-1,ig,中加西组)、健脾益气活血汤高、中、低剂量组(40,20,10 g·kg-1.d-1,ig),每组10只,以同龄同种系正常血压的京都种大鼠(WKY)10只作为WKY组(给予蒸馏水)。BP-98A型智能无创血压计监测大鼠尾动脉收缩压变化;治疗6周后腹主动脉取血测定血浆一氧化氮(NO)、前列环素(PGI2)、内皮源性超极化因子(EDHF)、血管紧张素Ⅱ(AngⅡ)、内皮素1(ET-1)浓度。结果:与SHR组收缩压(207.5±16.7)mmHg相比,培哚普利组(165.8±10.2)mmHg,中加西组(160.4±16.5)mmHg明显降低(P<0.01)。SHR组血浆NO(5.3±1.6)μmol·L-1,PGI2(27.2±6.3)ng·L-1、EDHF(106.7±3.2)ng·L-1浓度低于WKY组(33.8±7.5)μmol·L-1,(842.9±218.6),(347.0±44.4)ng·L-1,(P<0.01);培哚普利组、中加西组、中药高、中剂量组血浆NO,PGI2,EDHF浓度高于SHR组;SHR组大鼠血浆ET-1(77.1±13.9)ng·L-1,AngⅡ(21.0±2.6)μg·L-1浓度高于WKY组(20.8±1.9)ng·L-1,(1.1±0.3)ng·L-1,(P<0.05);培哚普利组、中加西组、中药高、中、低剂量各组大鼠血浆ET-1,AngⅡ浓度明显低于SHR组,(P<0.01)。结论:健脾益气活血汤可调控血管内皮舒缩因子的分泌与释放平衡,改善血管内皮功能,降低收缩压,减轻血压增高引起的血管内皮功能障碍。Objective：To study the influence of Jianpi Yiqi Huoxue decoction on systolic pressure and vascular endothelium vasomotor factors of spontaneously hypertensive rats（SHR）.Method：Sixty 24-week-old SHRs were randomly divided into six groups：SHR group（distilled water）,perindopril group（perindopril 0.4 mg·kg-1·d-1）,perindopril plus herb group（perindopril 0.4 mg·kg-1·d-1＋20 g·kg-1·d-1 of herb）,high,medium and low dose group（40,20,10 g·kg-1·d-1）.of Jianpi Yiqi Huoxue decoction and Wistar kyoto rats（WKY） group.The changes of systolic pressure were measured by BP-98A intelligent sphygmomanometer.After six weeks of treatment,the concentration of nitric oxide（NO）,prostacycline（PGI2）,endothelium derived hyperpolarizing factor（EDHF）,angiotensin Ⅱ（AngⅡ）,and endothelin（ET-1） in plasma of the rats were determined.Result：The systolic pressure of the perindopril group and the perindopril plus herb group was prominently decreased,compared with that of the SHR group（P0.01）.The concentration of NO,PGI2,and EDHF of the SHR group was lower than those of the WKY group,the perindopril group,the perindopril plus herb group,and the two herb dose groups（P0.01）.The concentration of ET-1,AngⅡ in the SHR was higher than those in the WKY group（P0.05）,and was significantly higher than those in the perindopril group,the perindopril plus herb group,and the three herb dose groups.Conclusion：The Jianpi Yiqi Huoxue decoction may improve functions of blood vessels,and reduce systolic pressure by regulating the balance between the secretion and release of vasomotor factors in vascular endothelium so as to modify the vascular endothelial function obstacles caused by hypertension.

关 键 词：健脾益气活血汤 自发性高血压大鼠 血管内皮舒张因子 血管内皮收缩因子 

分 类 号：R285.5[医药卫生—中药学]