活血化瘀中药对COPD血管重塑的干预作用  

Iintervention Effect of Activating Blood Circulation and Eliminating Stasis Chinese Herbals on Vessel Remolding of COPD

作　　者：张伟[1] 谷明明[2] 孙璐璐[2] 韩佳[2] 姚玉强[2] 

机构地区：[1]山东中医药大学附属医院,济南250011 [2]山东中医药大学,济南250014

出　　处：《中国实验方剂学杂志》2013年第8期254-257,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省自然科学基金项目(Y2006C114)

摘　　要：目的:观察慢性阻塞性肝病(COPD)模型大鼠应用活血化瘀药物后肺血管的病理变化,探讨活血化瘀中药对COPD血管重塑的干预作用。方法:用气管内注入脂多糖(LPS)及烟熏方法制作慢性阻塞性肺疾病模型。第29～42天,H组大鼠腹腔注射川青溶液8 mg·kg-1,每天1次,C组大鼠给予等体积生理盐水。于第6周处死3组大鼠,观察COPD模型大鼠应用活血化瘀药物后肺血管的病理变化。结果:C组和H组均有COPD特征性改变,但程度不同。H组血管平滑肌细胞轻度增生,管壁轻度增厚,血管平滑肌细胞厚度/血管壁厚度比值、血管壁面积/血管面积比值与N组、C组比较均有显著差异(P<0.01)。电镜下观察N组内皮细胞、基底膜、线粒体、板层小体正常;C组则出现明显变化,内皮细胞吞饮小泡增多,线粒体水肿或空泡变性,胞核有明显固缩倾向,毛细血管腔内见多形核白细胞堵塞;H组内皮细胞、基底膜、线粒体、板层小体基本正常,毛细血管腔内可见少量多形核白细胞。结论:活血化瘀药物能够延缓或者改善COPD大鼠模型的血管重塑,指导临床慢阻肺从瘀论治,根据其发展过程中的不同阶段并结合病情和体质进行辨证施治,灵活地运用活血化瘀药物,科学地看待活血化瘀在防治慢阻肺中的重要作用,以提高临床疗效。Objective：Observe the pulmonary vascular pathological change in chronic obstructive pulmonary disease（COPD） rat models of application for activating blood circulation and eliminating stasis drugs,explore the vascular remodeling of COPD.Method：Make COPD models by droping lipopolysaccharide（LPS） into tracheal and passive smoking method.From 29th to 42th days,group H are gave intraperitoneal injection of Chuanqing solution 8 mL·kg-1·day-1;group C are gave volumetric saline.Execute the three groups rats in sixth weeks,observe the pathological changes of pulmonary vessels in rat models applied activating blood circulation and eliminating stasis drugs.Result：Compared to group N there are characteristically changes in group C and H,but different in degree.Compared to group N and C,vascular smooth muscle cells of group H have mild hyperplasia,the wall was thickened slightly,the ratio of blood vessel wall/blood vessel wall and hemal wall area/vascular area are significantly（P0.01）.Under the electron microscopy observation,the endothelial cells,the basement membrane,mitochondria and shelf small body of group N are normal;group C appear obvious changes.The pinocytotic vesicles of endothelial cells increased,mitochondrion edema or vacuoles degenerate.The nuclei have become obvious tendency of becoming pyknotic.Many nucleation white blood cells are blocked in the capillary lumen;endothelial cells,the basement membrane,mitochondria and lamellar body in group H are basically normal.There are a few nucleation white blood cells in the capillary lumens.Conclusion：Traditional Chinese medicine for activating blood circulation and eliminating stasis can slow or improve the blood vessels remodeling of COPD.It guides the treat should accord to its different stages in the process of development and combined with illness,use the drugs for activating blood circulation and eliminating stasis flexibly,give a scientific understanding of the importance in control of COPD to improve the clinical effect.

关 键 词：慢性阻塞性肺疾病 活血化瘀中药 血管重塑 

分 类 号：R287[医药卫生—中药学]