多重耐药大肠埃希菌rmtB基因检测与整合子初步研究  

Primary study on rmtB and integron in multidrug-resistant of Escherichia coli

作　　者：魏秀丽 高迎春 魏秀青 苏梅 徐恩民 张传津 陈玲 

机构地区：[1]山东省兽药质量检验所,济南250022 [2]山东省茌平县医院,茌平252100

出　　处：《中国抗生素杂志》2013年第4期289-292,302,共5页Chinese Journal of Antibiotics

基　　金：十一五"国家科技支撑计划"重大项目"抗耐药菌中兽药的研制与开发(2008BADB4B04)"

摘　　要：目的对2010年临床分离的125株大肠埃希菌进行rmtB基因及整合子进行分析筛选。方法针对住院儿童大肠埃希菌采用KB法对氨基糖苷类药物及其他药物进行敏感性测定，并采用PCR方法扩增3种氨基糖苷类耐药基因和整合子。结果显示对12种药物耐药率达30％-75％。其中16SrRNA甲基化酶基因rrtB检出率12％，氨基糖苷乙酰转移酶基因aac（3）-II和氨基糖苷类核苷转移酶基因aadA1出率1．6％。rmtB阳性菌株携带I类整合子的检出率66．7％，主要携带了形秘J2＋aadA2＋DrfF、dfrAl7＋aada5两种排列形式。结论rmtB基因及I类整合子在大肠埃希菌中广泛存在，其在耐药性播散中发挥着重要的作用。Objective To investigate the distribution of methylase genes and Integron in 125 clinical isolates of Escherichia coli collected in 2010. Methods E. coli strains that isolated from hospital children were collected to test antibiotic susceptibility by K-B method. The aminoglycoside resistant genes and class I integron were amplified by PCR. Results 125 clinical isolates ofE. coli had obvious multidrug-resistance from 30%-75%. The 16S rRNA methylase genes were present in 12%, aac（3）- II and aadA1 were present in 1.6%. The isolates with rmtB were present in 66.7% also with class I integron, and the most popular gene cassette arrays were dfrA12＋aadA2＋orfF and dfrA17＋aadA5. Conclusion Drug resistance genes integrated by rmtB and class I integron were widespread in E. coll.

关 键 词：耐药性 大肠埃希菌 rmtB 整合子 

分 类 号：R378.1[医药卫生—病原生物学]