一株兼产淀粉酶和蛋白酶的烟碱降解菌的分离与鉴定  

Screening,isolation and identification of a high efficient nicotine-degrading strain with protease and amylase activities

作　　者：龙章德[1] 韦方立[2,3] 黄泰松[1] 韦建玉[1] 白森[1] 胡亚杰[1] 蔡联合[1] 申佩弘[2] 

机构地区：[1]广西中烟工业有限责任公司,广西南宁市530001 [2]广西大学生命科学与技术学院,广西南宁市530004 [3]广西民族师范学院化学与生物工程系,广西崇左市532200

出　　处：《工业微生物》2013年第2期33-37,共5页Industrial Microbiology

基　　金：广西中烟工业有限责任公司创新项目(20100037)

摘　　要：以烟碱为唯一碳源和氮源,从烟叶及植烟土壤中分离筛选出32株能够不同程度降解烟碱的菌株,复筛后发现一株具有强烟碱降解能力同时能够产生蛋白酶和淀粉酶的菌株A3,该菌株在含0.1%烟碱为唯一碳氮源的培养基中,,烟碱降解率为96.3%,在以0.2%的烟碱为唯一碳源和氮源的培养基中,30℃下摇床培养(转速为200 r/min)48 h,烟碱降解率为31.6%,同时蛋白酶和淀粉酶酶活分别达到42.6 U/mL和16.5 U/mL。对菌株A3进行常规形态学观察、生理生化特征研究、16S rDNA基因序列分析,将该菌株初步鉴定为烟草节杆菌,命名为Arthrobacter nicotianae GYC103。32 nicotine-degrading bacterial strains were separated and screened from tobacco and soil with selectivity cul- ture medium, in which nicotine was the sole source of carbon and nitrogen. Among them, strain A3 was the best one with strong ability to degrade nicotine, and it could secrete protease and amylase simultaneously. This strain was inoculated in the medium with 0.1% nicotine as carbon and nitrogen source, and incubated at 30~C for 18 h with reciprocal shaking （200 r/rain） ,the ratio of nicotine degradation was 96.3% ; and the ratio of degradation was 36.1% when the strain was inoculated in 0.2% nicotine for 48 h, meanwhile, the activities of pretease and amylase were 42.6 U/mL and 16.5 U/mL respectively. According to the normal morphology, physiological, biochemical characteristics and 16S rDNA gene se- quence, the strain A3 was identified as Arthrobacter nicotianae GYC103.

关 键 词：烟碱 烟草节杆菌 蛋白酶 淀粉酶 

分 类 号：S572[农业科学—烟草工业]