2007年～2010年H9N2亚型禽流感病毒分离株对SPF鸡的致病特性研究  

Pathogenic effects of H9N2 Avian Influenza Viruses between 2007 and 2010 on Chickens

作　　者：王守春[1] 张毅[2] 卢春晓[3] 王晓红[1] 徐守振[1] 王建琳[1] 李海英[1] 周顺[1] 王光[1] 郭妍妍[3] 毕玉海[4] 尹燕博[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国兽医药品监察所,北京海淀100081 [3]青岛即墨市畜牧兽医局,山东青岛266200 [4]中国科学院微生物研究所,北京海淀100101

出　　处：《中国兽医杂志》2013年第3期3-6,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(31272535);国家自然科学基金青年科学基金(31101830)

摘　　要：本研究对2007年～2010年我国H9N2亚型禽流感病毒分离株的鸡胚平均死亡时间(MDT)进行分析,发现自2008年下半年来分离的H9N2亚型禽流感病毒对鸡胚的致病性有增强的趋势,在此基础上,选择MDT在60.5～96.3h之间的9株病毒,比较其对SPF鸡的致病力。结果表明,对鸡胚致病性强的毒株,对SPF鸡的致病性也较强,能引起气管、肺、十二指肠、肾和脑等多器官的系统性感染,而且病毒在各组织器官的复制能力与致病性呈正相关。在感染SPF鸡后,所有毒株均呈现不同程度的排毒,而致病力较强毒株的感染组,排毒的比例更高,排毒时间也更长。这些数据表明,H9N2病毒在进化过程中,致病力发生了一定的变化,新近出现了一些对鸡致病力增强的毒株,这提示我们必须重视对H9N2病毒进化和变异的监测,加强对H9N2病毒的防控。During the surveillance of H9N2 subtype influenza viruses isolated between 20,07 to 2010, we found that the HgN2 viruses isolated after 2008 displayed higher virulence to embryonated eggs through the analysis,ofmean death time （MDT）. So, nine isolates with different MDT between 60.5 and 96.3 hours were selected to study their pathogenicity in Chickens, and the results showed that the pathogenicity was consistent with the MDT.The virus with higher virulence in embryonated eggs also showed higher pathogenicity in chickens, and caused systemic infection. Furthermore, the period of shedding of infectious challenge virus was longer, in the higher virulence virus infected group. All the data indicated that the virulence of some recently epidemic H9N2 viruses was enhanced, which suggested that the genetic and pathogenic variation of H9N2 viruses should be closely monitored.

关 键 词：H9N2亚型禽流感 致病性 SPF鸡 

分 类 号：S852.659.5[农业科学—基础兽医学]