基质细胞衍生因子-1对脂肪干细胞增殖、黏附的影响  

Effect of SDF-1 on Proliferation and Adhesion of Rat Adipose-Derived Stem Cells

作　　者：于彦领[1] 张新新[1] 林夏莲[1] 徐炜[1] 王明国[2] 

机构地区：[1]潍坊医学院口腔学教研室,山东潍坊261053 [2]济南市中心医院口腔科

出　　处：《潍坊医学院学报》2013年第1期11-13,共3页Acta Academiae Medicinae Weifang

摘　　要：目的以SD大鼠的脂肪干细胞(ADSCs)作为研究对象,在体外探讨研究基质细胞衍生因子(SDF-1)对大鼠源性的脂肪干细胞(ADSCs)增殖、粘附方面的影响。方法分别用不同浓度的SDF-1刺激ADSCs,观察48h,用MTT法检测不同浓度的SDF-1对ADSCs增殖的影响以及利用黏附模型评价不同浓度SDF-1对ADSCs粘附能力影响。结果 SDF-1剂量依赖性增强ADSCs的增殖能力在100mg/L时达到顶峰,与对照组相比,不同浓度SDF-1处理的ADSCs其粘附能力呈现浓度依赖性。结论 SDF-1可以促进ADSCs的黏附,但是只有在一定剂量范围内的SDF-1才可以促进ADSCs的增殖。Objective To investigate the effect of stromal cell-derived factor-1 on proliferation and adhesion of ADSCs using the rat adipose-derived stem cells（ADSCs） as the research objects. Methods Different concentration of SDF-1 were used to stimulate ADSCs respectively. After 48 hours, the proliferation of ADSCs was detected with MTI＇ method,and the adhesion ability of ADSCs was analyzed by microscope. Results SDF-1 increased the proliferation of ADSCs in a dose-dependent manner,but its role of promoting effect reached the top at 100mg/L and down-regulation at 200mg/L. Conclution SDF-1 can improve the proliferation of ADSCs in a certain concentration range. SDF-1 increased the adhesion of ADSCs in a dose-dependent manner.

关 键 词：基质细胞衍生因子 脂肪干细胞 增殖 黏附 

分 类 号：R318[医药卫生—生物医学工程]