婴幼儿人巨细胞病毒临床分离株包膜糖蛋白H基因型别研究  

Studies on genotype of human cytomegalovirus glycoprotein H from infantile clinical isolates

作　　者：郭珊[1] 于锰锰[1] 李革[1] 周华[1] 方峰[1] 舒赛男[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《中华儿科杂志》2013年第4期260-264,共5页Chinese Journal of Pediatrics

基　　金：湖北省自然科学基金（2010CDB05404）

摘　　要：目的研究婴幼儿人巨细胞病毒（HCMV）临床分离株包膜糖蛋白H（gH）的基因多态性，分析该基因不同型别在HCMV感染相关的不同疾病中的分布情况及其与不同疾病之间的关系。方法收集2010年5月至2012年10月华中科技大学同济医学院附属同济医院血HCMV—IgM、IgG阳性的不同疾病住院患儿的新鲜尿标本进行病毒分离，应用巢式PCR扩增结合限制性片段长度多态性及测序对临床分离株gH基因进行分型。结果102例临床分离株经巢式PCR扩增均为阳性（43例为婴儿肝炎综合征，38例为无黄疸型肝炎，13例为肺炎，7例为血小板减少性紫癜，1例为先天性CMV感染）。102例均得到gn基因分型结果，其中gH1型62株，占60．8％；gH2型40株，占39．2％，以gH1型为主，未见混合型和发现新的型别。gH基因型别在婴儿肝炎综合征（43株分离株中26例为gH1）、无黄疸型肝炎（38株分离株中25例为gH1）及肺炎（13株分离株中9例为gH1）中与总体分布趋势一致（χ2=0．357，P〉0．05），而在血小板减少性紫癜中以gH2型别为主，7株分离株中6例为gH2（χ2=6．083，P〈0．05）。结论本地区婴幼儿临床分离株gH基因型总体以gH1型占优势，在婴儿肝炎综合征、无黄疸型肝炎、肺炎等疾病中，gH基因型别分布与总体分布差异没有统计学意义，而在血小板减少性紫癜中以gH2型为主，提示gH型别可能与不同疾病表现存在一定的相关性。Objective To investigate the genetic polymorphism of human cytomegalovirus （HCMV） glycoprotein H （gH） from infantile clinical isolates, to analyze the genotypic distribution of gH in different diseases of HCMV infection and try to find the correlations between the diseases and genotypes. Method Fresh urine specimens were collected from the hospitalized children with different diseases whose blood HCMV-IgM and HCMV-IgG were positive. Virus was isolated from these specimens. Glycoprotein H of harvest clinical isolates was genotyped by nested-PCR combined with restriction fragment length polymorphism （RFLP）, the purified PCR products were digested by restriction endonuclease HhaI. The digested products were genotyped by polyacrylamide gel electrophoresis and silver staining. Classification and results of sequencing were compared. Result Totally 102 HCMV clinical isolates were obtained. Glycoprotein H gene of these clinical isolates （43 cases had infantile hepatitis syndrome, 38 cases had anicteric hepatitis, 13 pneumonia, 7 thrombocytopenic purpura, and 1 congenital CMV infection） were positive by nested-PCR, whose positive rate was 100%. The results showed that 62 strains were gill genotypes （60. 8% ）, while 40 strains were gH2 （39. 2% ）, mixed type or new genotype was not observed. In infantile hepatitis syndrome （26 clinical isolates were gill genotypes, 17 clinical isolates were gH2 genntypes）, anicteric hepatitis （25 were gill, 13 were gH2） and pneumonia （9 were gill, 4 were gH2）, the distribution of HCMV gH genotypes of infantile clinical isolates was consistent with the overall trend （ χ2 = 0. 357 ,P 〉 0. 05）. However , the gH2 was more common than gill in the clinical isolates of patients with thrombocytopenic purpura （6 were gH2, 1 were gH2, χ2 = 6. 083,P 〈 0. 05）. Conclusion Genotype 1 was the dominant genotype of glycoprotein H in HCMV clinical isolates from our hospital infants. Therewas no significant difference between the distribution of gH genotypes i

关 键 词：巨细胞病毒 基因型 包膜糖蛋白H 

分 类 号：R72[医药卫生—儿科]