机构地区:[1]昆明医科大学实验动物学部,云南昆明650031 [2]扬州大学兽医学院,江苏扬州225009
出 处:《Zoological Research》2013年第2期132-138,共7页动物学研究(英文)
基 金:云南省教育厅科学研究基金(2012C015);云南省科技基础条件平台建设计划(2006PT07-1);国家科技支撑计划(2009BAI83B02)
摘 要:解剖数据是实验动物主要的生物学特性指标。该文对实验室驯养树鼩(7~9月龄)的体尺、骨骼、乳头、肠道及脏器重量与系数等解剖学数据进行了测定与分析。31项解剖数据测量结果显示雌、雄个体间体高、右耳宽、回肠及结肠差异显著(P<0.05),体斜长、胸深、躯干长、左右两前肢长、右后肢长、左右两侧耳长、左耳宽、龙骨长、左右两侧胫长、十二指肠及空肠长等差异极显著(P<0.01)。以体长为因变量,尾长、躯干长、左前肢长、右前肢长、左后肢长及右后肢长等为自变量作逐步回归分析,回归方程为:体长=13.90+尾长×0.16。37项脏器及系数测定结果:雌雄间比较,体重、心、肺、脾、左肾、右肾、膀胱、左海马、右海马、左颌下腺、左甲状腺、右甲状腺重量差异极显著(P<0.01)。小肠、右颌下腺、左肾上腺之间差异显著(P<0.05);心、肺、胃、膀胱、小肠、大肠、脑、右海马、左肾上腺系数雌雄间差异极显著(P<0.01)。右肾、左海马、左颌下腺、右肾上腺、左右两侧甲状腺系数之间达到了显著性水平(P<0.05)。以动物体重为因变量,以主要脏器指标:心脏、肺、肝、脾、左肾、右肾、脑为自变量,作逐步回归分析,回归方程为:体重=62.73+左肾×79.213+心脏×24.09。实验室驯养树鼩不同性别对体尺、脏器及系数、肠道等解剖数据有一定影响,为树鼩实验动物化及人类疾病动物模型研究提供基础数据。Anatomical parameter values in tree shrews are major biological characteristic indicators in laboratory animals. Body size, bones and mammilla, organ weights, coefficient intestinal canal and other anatomical data were measured and analyzed in laboratory domesticated tree shrews (7 to 9 months of age). Measurement of 31 anatomical parameters showed that body height, width of the fight ear, ileum and colon had significant differences between males and females (P〈0.05). Highly significant differences were also found in body slanting length, chest depth, torso length, left and fight forelimb length, fight hind limb length, left and fight ear length, left ear width, keel bone length, left and fight tibia length, duodenum and jejunum (P〈0.01). With body length as the dependent variable, and tail length, torso length, fight and left forelimb length, and left and fight hind limb length as independent variables for stepwise regression analysis, the regression equation for body length = 13.90 + tail length × 0.16. The results of 37 organs weights between female and male tree shrews showed very significant differences (P〈0.01) for weight of heart, lungs, spleen, left and fight kidney, bladder, left and fight hippocampus, left submandibular gland, and left and fight thyroid gland, as well as significant (P〈0.05) differences in the small intestine, fight submandibular gland, and left adrenal gland. The coefficient of heart, lung, stomach, bladder, small and large intestine, brain, fight hippocampus, and left adrenal gland showed highly significant differences (P〈0.01), while differences in the fight kidney, left hippocampus, left submandibular gland, fight adrenal gland, and left and fight thyroid gland were significant (P〈0.05). With animal weight as the dependent variable and indicators of heart, lung, liver, spleen, left and fight kidney and brain as independent variables for stepwise regression analysis, the regression equation showed that weight = 62.73 + left kidney× 79.21
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