人心房利尿钠肽减轻油酸诱导大鼠急性肺损伤炎症反应实验研究  

Experimental study on human atrial natriuretic peptide to alleviate oleic acid-induced acute lung injury inflammatory response

作　　者：朱耀斌[1] 范祥明[1] 李晓锋[1] 李志强[1] 刘爱军[1] 李刚[1] 刘迎龙[1] 

机构地区：[1]首都医科大学附属北京安贞医院小儿心脏中心,北京市100029

出　　处：《中华实用诊断与治疗杂志》2013年第4期328-329,332,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：首都医科大学基础-临床科研合作基金(11JL50);国家自然科学基金(30471721);国家自然科学基金(30670928);国家自然科学基金(81070055);科研基地-科技创新平台(PXM2011_014226_07_000060);北京市自然科学基金资助项目(7112046;7122056);北京市医药产品和技术重大项目培育研究项目(Z101107050210020);北京市科委首都市民健康项目(Z111100074911001);北京市卫生系统高层次卫生技术人才培养计划(领军人才2011-1-4)

摘　　要：目的探讨人心房利尿钠肽(human atrial natriuretic peptide,hANP)对油酸诱导大鼠肺部炎症反应的影响。方法 18只SD大鼠,随机分为3组各6只,麻醉完成,建立呼吸机、心电监测,稳定30min后对照组单纯给予生理盐水1mL/kg,肺损伤组给予油酸0.1mL/kg,油酸-hANP组给予油酸0.1mL/kg,并于油酸注射30min后静脉注射hANP0.1g/(kg·min);油酸注射6h后,3组采用定量ELISA检测血浆及肺组织中白细胞介素-1β,-6,-10及肿瘤坏死因子-α水平。结果肺损伤组血浆与肺组织白细胞介素-1β,-6,-10与肿瘤坏死因子-α水平明显高于对照组与油酸-hANP组(P<0.05);油酸-hANP组高于对照组(P<0.05)。结论 hANP可减轻油酸诱导大鼠肺部炎症反应。Abstract： Objective To investigate the influence of human atrial natriuretic peptide （hANP） on oleic acid-induced pulmonary inflammatory response in rats. Methods Eighteen SD rats were randomly divided into 3 groups, with 6 rats in each group. After anesthesia, all rats received respirator and ECG monitoring. Thirty minutes after stabilization, control group was treated with 1 mL/kg saline, lung injury group was treated with 0.1 mL/kg oleic acid, and oleic acid-hANP group was treated with 0.1 mL/kg oleic acid followed with intravenous injection of 0.1 g/（kg · min） hANP 30 minutes after injection of oleie acid. Six hours after injection of oleie acid, quantitative ELISA technique was used to detect plasma and tissue interleukin-1β, interleukin-6, interleukin 10 and tumor necrosis factor-α levels in all these three groups. Results The levels of interleukin- 1β, interleukin-6, interleukin-10 and tumor necrosis factor-α in plasma and tissue were higher in lung injury group that those in control group and oleic acid hANP group （P〈0.05）, and higher in oleic acid- hANP group than those in control group （P 〈 0.05 ）. Conclusion hANP can alleviate oleic acid-induced lung inflammatory response in rats.

关 键 词：肺损伤 油酸 人心房利尿钠肽 

分 类 号：R725.4[医药卫生—儿科]