微重力环境对大鼠血浆蛋白质谱的影响  

Effects of Simulated Weightlessness on Proteomic Profiles of Plasma in Rats

作　　者：纪安来 张军[1] 周金莲[1] 罗治文[1] 吉敏[1] 杨鹤鸣[1] 李成林[1] 王平[1] 崔彦[1] 

机构地区：[1]解放军第306医院,北京100101

出　　处：《生物技术通讯》2013年第2期230-234,共5页Letters in Biotechnology

基　　金：全军医学科研"十二五"重点项目(BWS11J051)

摘　　要：目的：探讨模拟失重环境下大鼠血浆蛋白质组变化特征。方法：健康成年雄性Wistar大鼠88只，按模拟失重时相随机分为11组，分别为6h、12h、1d、2d、3d、5d、1周、2周、3周、4周及0h组（对照组）。采用尾悬吊法建立模拟失重动物模型，实验结束时取动物静脉血，利用表面增强激光解吸电离飞行时间质谱（SELDI—TOF—MS）技术及MB—WCX磁珠检测大鼠静脉血浆蛋白质谱，应用Ciphergen Protein Chip Software3．2．0和Biotaarker Wizard3．1．0软件分析数据。结果：发现18个重力敏感蛋白，其中在模拟失重早期，相对分子质量较小的6个蛋白的表达呈上调趋势，而相对分子质量较大的12个蛋白的表达则逐渐下调；在模拟失重后期（悬尾2～3周后），上述蛋白的表达均呈回归趋势。结论：模拟失重环境对大鼠静脉血浆蛋白质谱产生明显影响，研究重力敏感蛋白对进一步揭示失重对机体的影响及机制具有重要意义，并对医监医保可能有一定的价值。Objective： To investigate the effects of the simulated weightlessness on the proteomic profiles of plas- ma in rats. Methods： Eighty-eight male adult Wistar rats were randomly divided into 11 groups according to the durations of simulated weightlessness, suspended for 6 h, 12 h, 1 d, 2 d, 3 d, 5 d, 1 week, 2 week, 3 week, 4 week and 0 h（coutrol）. Tail-suspension technique was used to simulate the weightlessness animal model. The rats were sacrificed and the venous blood samples were taken in each group at the end of the experiment. The pro- teomic profiles of the blood plasma were detected by the surface-enhanced laser desorption ionization time of flight mass spectrometry（SELDl-TOF-MS） and MB-WCX beads. All data were analyzed with the Ciphergcn Pro- tein Chip Software 3.2.0 and Biomarker Wizard 3.1.0. Results： Eighteen peaks of gravity-sensitive proteins were discovered, and 6 proteins with low molecular weight were up-regulated in the early phase of the simulated weight- lessness, while 12 proteins with higher molecular weight showed downward trend on the contrary. Interestingly, a reversion to the standard levels was noticed in all the proteins of the rat blood plasma in the late phase（2 to 3 week after tail-suspension） of the simulated weightlessness. Conclusion： Simulated weightlessness could induce al- terations of proteomic profiles of rat plasma, and detection of the gravity-sensitive proteins could provide useful messages to disclose the mechanism of weightlessness impacts on the body and may also be meaningful in the as- tronaut medical surveillance.

关 键 词：模拟失重 血浆蛋白 蛋白质组 表面增强激光解吸电离飞行时间质谱 

分 类 号：Q959.837[生物学—动物学]