西兰花再生体系的建立与优化  

Establishment and Optimization of Broccoli Regeneration System

作　　者：刘煜[1] 王红波[1] 王传琦[1] 李晶[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《生物技术通讯》2013年第2期238-240,共3页Letters in Biotechnology

基　　金：黑龙江省留学归国及海外学者合作基金

摘　　要：目的:以西兰花无菌苗为材料,对影响西兰花再生的各种因素进行研究,建立并优化西兰花的再生体系。方法:用组织培养法,对西兰花的外植体进行诱导生芽、生根。结果:不同品种的再生率差别较大,珠绿和青秀两个品种的再生率达91%以上,而鼎丰一号仅为80.3%;培养6 d的下胚轴分化能力最强;当培养基中添加3 mg/L 6-BA和0.2 mg/L IAA时,下胚轴的分化率最高,玻璃化程度也降到最低;当培养基中添加0.2 mg/L IBA或0.2 mg/L NAA时,可成功诱导不定芽生根。结论:建立并优化了西兰花再生体系,为西兰花遗传转化体系的建立奠定了基础。Objective： Using axenic seedlings as explants, several factors influencing regeneration rate of broccoli were studied and a successful tissue regeneration system was established and optimized. Methods： Using tissue culture mesthod to induce the explant of broccoli yielding bud and root. Results： The study showed that the geno- type influenced regeneration rate greatly. The regeneration rate of Zhu-lv and Qing-xiu was above 91% while Ding- fengl is 80.3%; differentiation ability of hypocotyls which had been cultured 6 days was the strongest. When the medium contained 3 mg/L 6-BA and 0.2 mg/L IAA, the differentiation rate of hypocotyls reached the highest lev- el and the degree of vitrification was the lowest. In the medium contains 0.2 mg/L IBA or 0.2 mg/L NAA, adventi- tious root could be successfully induced from the adventitious buds. Conclusion： Broccoli regeneration system has been successfully established, which laid the foundation for the establishment of broccoli genetic transformation sys- tem.

关 键 词：西兰花 组织培养 再生体系 

分 类 号：Q945[生物学—植物学]