不同荧光染色方法检测HMC毒素诱导处理玉米B37根冠细胞凋亡  

Different Fluorescent Staining Methods for Inducing of Apoptosis in Homocaryon with B37 of Maize by Helminthosporium maydis Race C(HMC) Toxin

作　　者：马春红[1] 李秀丽[1,2] 董文琦[3] 张红心[4] 戴志刚 王立安[2] 贾银锁[1] 

机构地区：[1]河北省农林科学院遗传生理研究所/河北省植物转基因中心,石家庄050051 [2]河北师范大学生命科学学院,石家庄050016 [3]河北省农林科学院科技处,石家庄050051 [4]唐山师范学院,河北唐山063000 [5]湖北省土壤肥料工作站,武汉430064

出　　处：《玉米科学》2013年第2期98-101,共4页Journal of Maize Sciences

基　　金：河北省自然科学基金(C2011301010);河北省高层次人才"三三三人才工程"项目(A2010003028);河北省科技计划项目(13396401D);科技部国际合作项目(2006DFB02480);农业部948重点项目[2011-G1(3)-07]

摘　　要：采用吖啶橙(AO)与溴化乙啶(EB)荧光双染色以及Hoechst 33258荧光染色法对HMC毒素诱导玉米B37-C、B37-N根冠细胞凋亡率进行检测。结果表明,采用AO/EB双染色后,当HMC毒素浓度为50、100、150μg/mL,处理7 h时B37-C根冠细胞凋亡率分别为24.8%、58.2%、70.2%,B37-N仅为11.0%、25.7%和36.6%;当浓度为150μg/mL,处理时间4 h时B37-C根冠细胞最大凋亡率为62.3%,B37-N为25.8%。经Hoechst 33258染色后,HMC毒素浓度为50、100、150μg/mL,处理7 h时B37-C的根冠细胞凋亡率分别为32.5%、58.7%、74.5%,B37-N仅为7.5%、22.3%和30.7%;HMC毒素浓度为150μg/mL,处理4 h时B37-C的细胞凋亡率为62.3%,B37-N为19.3%。AO and EB fluorescent double staining and Hoechst 33258 fluorescent staining methods were used to detach the apoptosis of root cap cells. Detection of apoptosis and the change of its ratio induced by HMC toxin in the root cap cells detached from a pair of homokaryon B37-C and B37-N inbred line of maize. In contrast, the ratios of apoptosis of B37-C were 24.8%, 58.2%, 70.2%, and B37-N were 11.0%, 25.7%, 36.6% by AO and EB staining, after HMC-toxin with 50, 100, 150 μg/mL treated for 7h. The max ratios of apoptosis of B37-C and B37-N stained by AO and EB were 62.3% and 25.8%, respectively, after HMC toxin with 150 μg/mL treated for 4 h. And the ratios of apoptosis of B37-C were 32.5%, 58.7%, 74.5% and B37-N were 7.5%, 22.3%, 30.7% by Hoechst 33258 staining, after HMC-toxin with 50, 100, 150 μg/mL treated for 7 h. The max ratios of apoptosis of B37-C and B37-N stained by Hoechst 33258 were 62.3% and 19.3%, respectively, after HMC toxin with 150 μg/mL treated for 4 h.

关 键 词：玉米 小斑病菌C小种 毒素 细胞凋亡 荧光染色 

分 类 号：S513.01[农业科学—作物学]