推拿法引发人骨骼肌细胞生物学效应的机制探讨  

Biological Effect of Rolling Manipulation on Human Skeletal Muscle Cells

作　　者：林清[1,2] 张宏[1] 赵谦 牛坤[4] 张国辉[1] 王勇丽[1] 屈庆[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]苏州卫生职业技术学院,江苏苏州215009 [3]兖州市中医院,山东兖州272100 [4]海南医学院,海南海口571101

出　　处：《上海中医药大学学报》2013年第2期81-84,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81173359);上海市教委科研创新项目(09YZ138)

摘　　要：目的:探索推拿法力学作用引发人骨骼肌细胞生物学效应的机制。方法:采用细胞培养技术,建立人骨骼肌细胞损伤模型后,将正常细胞和损伤细胞各自分为空白对照组、推拿法组、推拿法+维拉帕米组、静态压力组和静态压力+维拉帕米组。分别对各组细胞进行推拿法样刺激或静压刺激,持续时间10 min。其后对各组细胞的超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、肌酸激酶(CK)活力进行检测。结果:推拿法可以提高人骨骼肌细胞内SOD活力,降低细胞内MDA含量以及细胞上清中的CK活力;在加入钙离子拮抗剂———维拉帕米后,各项指标的变化均受到明显的影响。结论:推拿法的力学作用主要通过影响人骨骼肌细胞中钙离子的信号通路,从而激发损伤细胞修复的生物学效应。Objective： To explore the biological mechanism of rolling manipulation on human skeletal muscle cells.Methods： The models were established by cell culture technology.The normal cells and damaged cells were divided into control group,rolling manipulation group,rolling manipulation-Verapamil group,static pressure group and static pressure-Verapamil group.Rolling manipulation and static pressure were applied in the corresponding group for 10min.Then the vitality of SOD and CK,and the content of MDA were tested in each group.Results： Rolling manipulation can increase the vitality of CK,decrease the vitality of CK and content of MDA;all the indexes were changed obviously after adding the Verapamil.Conclusion： The rolling manipulation can stimulate the biological effects on human skeletal muscle cells through influencing the calcium signaling pathway.

关 键 词：推拿 法 骨骼肌细胞 生物学效应 超氧化物歧化酶 丙二醛 肌酸激酶 

分 类 号：R244.1[医药卫生—针灸推拿学]