HPLC-ICP-MS法分析测定药用植物中的形态汞  

Determination of mercury species in chinese herbs by HPLC-ICP-MS

作　　者：宋旭 孔维恒[2] 高峰[2] 李晶[2] 朱平丽[3] 王金花[2] 

机构地区：[1]北京市怀柔第一医院,北京101400 [2]北京出入境检验检疫局,北京100026 [3]珀金埃尔默仪器(上海)有限公司,上海201203

出　　处：《现代仪器与医疗》2013年第2期1-4,共4页Modern Instruments & Medical Treatment

基　　金：北京市科技计划项目资助(项目编号:D08050200310805)

摘　　要：确定针对连翘、红花、番泻叶、西洋参、川穹、当归等药用植物中的汞形态（甲基汞、乙基汞、二价汞离子）超声提取样品前处理方法，采用phenomenex C18液相色谱柱进行分离，ICP-MS检测。样品回收率72.83%~98.25%，精密度（RSD）（n=6）均小于15%，甲基汞、乙基汞、二价汞的检出限分别为0.125mg/kg，0.250mg/kg，0.125 mg/kg。该方法具有灵敏度高、干扰小、样品前处理简单等优点，可用于药用植物中汞形态的筛查和测定。The pre-treatment method of ultrasonic for mercury speciation （Methylmercury, Dimercurion , Ethylmercury） of forsythia, safflower, senna, gen-seng, Rhizoma Chuanxiong, angelica in Chinese herbs were established. Mercury speciation were separated using the phenomenex C18 column of high performance liquid chromatography with inductively coupled plasma mass spectrometry. The recoveries of three mercury speciation were 72.83%~98.25%. Relative standard deviations （n=6） were 〈15%. Limit of detections for Methylmercury, Dimercurion and Ethylmercury were 0.005mg/L, 0.010mg/L, 0.005mg/L respectively. The advantages of the method were easy sample extraction and separation , less interference and high sensitivity.

关 键 词：高效液相色谱-电感耦合等离子体质谱 汞形态分析 

分 类 号：R284.1[医药卫生—中药学] O67[医药卫生—中医学]