猪流行性腹泻病毒S蛋白抗原表位区基因克隆、分析及原核表达  

Cloning and expression of antigen epitopes of spike protein of porcine epidemic diarrhea virus

作　　者：肖建雄[1] 唐志玲[1] 罗满林[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《广东畜牧兽医科技》2013年第2期17-20,共4页Guangdong Journal of Animal and Veterinary Science

摘　　要：从患流行性腹泻的病猪小肠中分离猪流行性腹泻病毒(PEDV),设计一对引物用于扩增PEDV S蛋白的抗原表位区,将扩增产物克隆到原核表达载体pET-32a,构建了重组表达质粒pET32a-PEDV-S1;在IPTG的诱导下表达,经SDS-PAGE分析,West ern bl ot t i ng鉴定,结果表明该抗原表位区蛋白得到了重组表达。该研究可为PEDV抗原表位区的相关研究提供参考。The porcine epidemic diarrhea virus （PEDV） was isolated from small intestine of pigs with diarrhea, and a pair of specific primers was desiged and synthesized to amplify antigenic region of the spike protein gene by reverse transcription polymerase chain reaction （RT-PCR）. Then the amplified fragment was cloned into prokaryotic expression vector pET32a, gained a recombinant expression plasmid pET32a-PEDV-S1.The recombinant protein was expressed and identified by SDS-PAGE and Western blotting.The results could be helpful to the related research of PEDV.

关 键 词：PEDV S蛋白 抗原表位区 原核表达 

分 类 号：S852.69[农业科学—基础兽医学]