犬瘟热病毒荧光定量RT-PCR检测方法的建立及初步应用  

Real-time RT-PCR method for detection of canine distemper virus and application

作　　者：余绍华[1] 罗满林[1] 苏力[2] 蔡勤辉[2] 陈武[2] 陈虹[3] 黄晓卉[3] 苏乔[3] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广州动物园,广东广州510070 [3]中山大学附属第一医院,广东广州510080

出　　处：《广东畜牧兽医科技》2013年第2期35-39,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省科技基础条件建设项目(2009B060300004)

摘　　要：根据犬瘟热病毒的核衣壳蛋白(N)基因保守序列,设计特异性引物,建立犬瘟热病毒荧光定量RT-PCR检测方法。试验结果表明,该方法敏感性比普通RT-PCR高出100倍,重复性良好,变异系数在1.01%以下,对犬的常见病原核酸提取物检测均为阴性,证明特异性良好。对32份临床样本进行检测,27份为阳性,高于普通RT-PCR方法。试验结果表明该方法可用于犬瘟热感染情况的检测。On the basis of conserved sequence of nucleocapsid protein（N） gene of the canine distemper virus （CDV）, a pair of specific primers was designed to establish real-time RT-PCR method for the detection of CDV. The results showed that the sensitivity of this method was 100 times higher than that of RT-PCR. This method has good repetitiveness. The coefficient of variation was less than 1.01%. Pathogens associated with canine diseases were not amplified by this method, demonstrating its excellent specificity. In clinical applications, 27 positive samples were detected out of 32 clinical specimens. These results indicated that the real-time RT-PCR can be used for the detection of canine distemper virus.

关 键 词：犬瘟热病毒 核衣壳蛋白(N)基因 荧光定量RT-PCR 

分 类 号：S85[农业科学—兽医学]