复方马钱子的抗肿瘤作用探讨  

The study on the anti-tumor effect of compound nux vomica

作　　者：刘迎辉[1] 林海红[1] 杜钢军[1] 

机构地区：[1]河南大学药学院药物研究所,河南开封475004

出　　处：《河南大学学报（医学版）》2013年第1期28-31,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金项目(81173094);河南省科技厅重点攻关项目(112101310308);河南省高校青年骨干教师项目(2010GGJS-025);河南大学省部共建项目(SBGJ090704)

摘　　要：目的探讨复方马钱子的抗肿瘤作用。方法采用小鼠H22肝癌皮下移植肿瘤模型,通过检测原位肿瘤、免疫器官指数及Na+、K+、Cl-、葡萄糖含量,评价复方马钱子的毒性及抗肿瘤作用。采用MTT法检测复方马钱子对淋巴细胞和HCT-116癌细胞增殖的影响。结果复方马钱子在实验剂量下,与模型对照组比较,治疗组的肿瘤生长减慢,荷瘤小鼠免疫器官指数降低,血清中K+、Cl-、葡萄糖含量显著增加,Na+浓度不受影响。体外实验表明,复方马钱子对HCT-116癌细胞有一定的抑制作用,但对淋巴细胞增殖无影响。结论复方马钱子有一定的抗肿瘤作用,可调节体内电解质、葡萄糖含量使趋于正常。Objective To investigate the anti-tumor effect of compound nux vomica.Methods H22 hepatic cancer subcutaneously transplanted model was used to evaluate the anti-tumor effect of compound nux vomica by detecting situ tumor size,immune organ index,Na＋,K＋,Cl-,and glucose levels.MTT assay was used to observe effect of compound nux vomica on lymphocyte and HCT 116 cancer cell proliferation.Results Compared with the model group,in the compound nux vomica-treated mice,the tumor grew more slowly,the immune organ index decreased obviously,K＋,Cl-,glucose in serum significantly increased but Na＋ kept unchanged.In vitro,compound nux vomica suppressed HCT-116 cancer cell proliferation but had no effect on lymphocytes.Conclusion Compound nux vomica have anti-tumor effect and can regulate electrolytes and glucose levels to keep balanced.

关 键 词：复方马钱子 抗肿瘤 MTT 离子 葡萄糖 

分 类 号：R285.5[医药卫生—中药学]