人工发酵虫草菌丝体干粉对链脲佐菌素诱导糖尿病大鼠心脏NF-κB和TGF-β1表达影响的研究  

Therapeutic effect of artificial fermented cordyceps mycelium on the expressions of NF-κB and TGF-β1 in the cardiac muscle of STZ diabetic rats

作　　者：谷优优[1] 高桦[1] 吴梅筠[1] 张鑫[1] 张鹏[1] 赵相卓[1] 马中书[1] 邱明才[1] 

机构地区：[1]天津医科大学总医院内分泌科,300052

出　　处：《中国糖尿病杂志》2013年第4期357-362,共6页Chinese Journal of Diabetes

摘　　要：目的观察人工发酵虫草菌丝体干粉(CM)对糖尿病大鼠心肌核因子-κB(NF-κB)和转化生长因子-β1(TGF-β1)表达的影响。方法健康雄性SD大鼠53只,鼠龄8周,体重(220±20)g。采用数字表法将大鼠随机分为正常对照组(NC,n=10)、单纯糖尿病组(n=10)、CM小剂量组[n=12;CM 0.6g/(kg.d)灌胃]、CM中剂量组[n=11;CM 2.5g/(kg.d)灌胃]、CM大剂量组[n=10;CM 5g/(kg.d)灌胃]。单次尾静脉注射STZ(45mg/kg)造模,成模8周后处死大鼠。采用免疫组织化学法、RT-PCR法检测心肌NF-κB、TGF-β1蛋白和mRNA的表达水平。结果单纯糖尿病组心肌NF-κB、TGF-β1蛋白和mRNA表达均上调(P<0.05),CM干预可下调NF-κB、TGF-β1蛋白和mRNA的异常表达(P<0.05)。结论心肌组织中,NF-κB和TGF-β1表达的改变可能参与了糖尿病心肌病(DC)的发生,CM可能通过调节NF-κB和TGF-β1在心肌的表达发挥对心肌的保护作用。Objective To observe the effect of artificial fermented cordyceps mycelium （CM） on the expressions of NF-κB and TGF-β1 in the cardiac muscle of streptozocin-induced diabetic rats. Methods A total of 53 healthy male SD rats, aged 8 Weeks, weighted（220±20）g, were randomized into normal control group （n = 10）, diabetic group （n = 10）, and CM group In = 12, 0.6 g/（kg .d） ; n = 11, 2. 5 g/（kg . d）; n=10, 5 g/（kg . d）]. Tail vein injection with streptozotocin 45 mg/kg was adopted to produce the diabetic rat models. Diabetic rats were sacrificed after 8 weeks. The expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle were determined by using immunohistoche-mistry staining and reverse transcription-polymerase chain reaction （RT-PCR） method. The data were analyzed by using one-factor variance analysis. Results The expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle of diabetic rats were significantly increased （P〈0. 05）. The intervention with cordyceps mycelium could decrease the expressions of NF-κB and TGF-β1 proteins and mRNA （P〈 0.05）. Conclusion The changes in the expressions of NF-κB and TGF-β1 in the myocardial tissue may be involved in the occurrence of diabetic cardiomyopathy. Cordyceps mycelium may play its role on myocardial protection through regulating the expressions of NF-κB and TGF-β1in the myocardium.

关 键 词：糖尿病 糖尿病心肌病 核因子-ΚB 转化生长因子-β1 人工发酵虫草菌丝体干粉 大鼠 

分 类 号：R587.1[医药卫生—内分泌]