秋水仙素条件优化及其对牛卵细胞c-mos基因表达水平的影响  

Optimal conditions of demecolcine affecting the expression level of c-mos gene in bovine oocytes

作　　者：任久生[1,2] 邓琼[1,2] 刘慧玉[1,2] 徐靖博[1,2] 柳思源[1,2] 李付娟[1,2] 俞先峰[2] 张嘉保[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《中国兽医学报》2013年第4期636-640,共5页Chinese Journal of Veterinary Science

基　　金：现代农业产业技术体系建设专项资金资助项目(CARS-38)

摘　　要：为探讨秋水仙素对牛卵母细胞产生细胞膜突起的最适条件与对减数分裂纺锤体组装起重要调控作用的母源基因c-mos表达量的影响,设计如下试验:比较试验组(Deme+sucrose处理)与对照组1(Deme单独处理)和对照组2(sucrose单独处理)的卵母细胞细胞膜突起率变化。用荧光定量检测最适合秋水仙素浓度处理的卵母细胞与空白组和对照组母源基因c-mos基因表达量的差异。结果表明,用0.6mg/L秋水仙素+0.05mol/L蔗糖处理牛卵母细胞1h细胞膜突起率达72.0%,要显著高于对照组1(13.0%)和对照组2(P<0.05);0.6mg/L秋水仙素浓度优于其他质量浓度(P<0.05)。秋水仙素加蔗糖处理的卵母细胞的母源基因c-mos表达高于蔗糖处理组和空白组c-mos基因的表达(P<0.05)。To investigate the optimal conditions of demeclocine which induces the membrane protrusions of bovine oocytes and the effect of c-mos; expression level which is a kind of maternal gene and plays an important role in the assemble of spindle. In this study, comparing with the changes of bovine oocytes membrane protrusions among the experimental group（Demeq-sucrose）, control groups（Deme）and untreated groups （sucrose）. Selecting optimal condition of demecoline and detecting the expressing level of maternal gene c-mos in the different treatment. The incidence of protrusions reaches 72.0; with the treatment of demeclocine and sucrose for 1 h. It is significantly higher than Demey and sucrose group （P〈0.05）. The results shows that the treatment of 0.6 mg/L demeclocine is better than the other concentrations. The maternal gene c-mos gene expression in the groups with the treatment of demeclocine and sucrose is higher than the treatment of sucrose groups and untreated groups （P〈0.05）.

关 键 词：秋水仙素 牛卵母细胞 c—mos 

分 类 号：S852.1[农业科学—基础兽医学]