促性腺激素对输卵管黏膜上皮细胞NGF及TrkA表达影响  

Gonadotropin affects the expression of nerve growth factor(NGF) and its receptors(TrkA) in oviduct epithelial cells

作　　者：刘立文[1,2] 马永和[2] 李万宏[2] 王春强[2] 

机构地区：[1]河北北方学院动物科技学院,河北张家口075131 [2]吉林大学动物科学学院,吉林长春130062

出　　处：《中国兽医学报》2013年第4期641-644,共4页Chinese Journal of Veterinary Science

基　　金：国家转基因生物新品种培育重大专项资金项目(2011ZX08007-003)

摘　　要：利用荧光定量PCR和ELISA技术研究了LH和FSH对输卵管黏膜上皮细胞表达NGF及其受体TrkA的影响。结果显示:FSH能促进NGF及其受体TrkA的mRNA的表达(P<0.05);LH能促进NGF mRNA的表达(P<0.05),也能促进TrkA mRNA的表达,但差异不显著(P>0.05)。ELISA结果表明,添加LH和FSH后,NGF的表达量有所增加,但是差异不显著(P>0.05)。这说明促性腺激素LH和FSH对NGF和TrkA的表达有影响。the effects of gonadotropin on the expression of NGF and TrkA in oviduct epithelial cells were investigated with fluorescence quantitative-PCR and ELISA. The results of fluorescence quantitative-PCR indicated that the expression level of NGF mRNA was significantly higher than that of the control when treated with LH（10 or 20 mg/L） （P〈0.05） ;although the level of TrkA mRNA increased,no statistical difference was observed（P〉0.05）. The expression level of NGF mRNA in oviduct epithelial cells cultured with FSH at the concentration of 20 mg/L was signifi- cantly higher than that of the control（P〈0.05） ,and the amount of TrkA mRNA also significantly increased when treat with FSH（10 or 20 mg/L）. The results of ELISA showed that the expression level of NGF protein was higher in the LH and FSH groups than that of the control,but no statistical difference（P〉0. 05 ）. The results showed that the gonadotropins could affect the expression of NGF and TrkA in oviduct epithelial cells.

关 键 词：输卵管 神经营养因子 促性腺激素 

分 类 号：S852.1[农业科学—基础兽医学]