清开灵注射液中14种胆汁酸的HPLC-ESI-MS/MS快速鉴定  

Rapid identification of 14 bile acids contained in Qingkailing injection by HPLC-ESI-MS/MS

作　　者：张加余[1,2] 张倩[1] 张红霞[1] 董鲁艳[1] 卢建秋[2] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学科研实验中心,北京100029

出　　处：《中国中药杂志》2013年第7期990-994,共5页China Journal of Chinese Materia Medica

基　　金：国家重大新药创制专项(2010ZX09502-002);中药注射剂安全性研究技术平台建设项目

摘　　要：目的:研究清开灵注射液中胆汁酸类成分的ESI-MS/MS裂解规律及其快速识别方法。方法:采用HPLC-ESI-MS/MS技术研究5种胆汁酸类成分的ESI-MS/MS裂解规律,并对8个生产厂家清开灵注射液中的胆汁酸类成分进行快速鉴别。结果:5种胆汁酸类成分具有相似的ESI-MS/MS裂解特征;利用这些特征从清开灵注射液中快速鉴定了14个胆汁酸类成分;其中11个为8个厂家注射液共有成分,3个存在于个别厂家产品中。结论:利用HPLC-ESI-MS/MS技术可以从复杂物质体系中快速鉴定胆汁酸类成分,并为中药复方制剂的复杂物质基础阐明与质量标准提高提供了有效的方法。To investigate ESI-MS/MS fragmentation pattern of bile acids contained in Qingkailing injection, and the rapid identification method. Method： HPLC-ESI-MS/MS technology was adopted to investigate ESI-MS/MS fragmentation pattern of five bile acids contained in Qingkailing injection, and rapidly identify bile acids contained in Qingkailing injection from eight differ- ent manufacturers. Result- 5 bile acids showed similar ESI-MS/MS fragmentation patterns, based on which 14 bile acids were rapidly separated from Qingkailing injection. Among them, 11 were found in injections of all of the manufacturers, and the rest three were found in individual manufacturers. Conclusion： HPLC-ESI-MS/MS is used to rapidly identify bile acids from complex material systems, which provides an effective method for interpreting the complex material base of compound traditional Chinese medicine preparations and enhance the quality standards.

关 键 词：清开灵注射液 胆汁酸 液质联用 ESI—MS MS裂解规律 快速鉴定 

分 类 号：R286[医药卫生—中药学]