基于大孔吸附树脂纯化技术比较不同产地铁棒锤中总生物碱与乌头碱的含量  

Comparison of content of total alkaloids and aconitine in Aconitum szechenyianum from different producing areas on basis of macroporous adsorption resin purification technology

作　　者：林丽[1,2] 魏学明 王延惠[1] 晋玲[1,2] 贺正阳[1] 杜弢[1,2] 陈红刚[1,2] 

机构地区：[1]甘肃中医学院药学系,甘肃兰州730000 [2]甘肃省高校中藏药化学与质量研究重点实验室,甘肃兰州730000 [3]甘肃奇正藏药有限公司,甘肃兰州730100

出　　处：《中国中药杂志》2013年第7期995-999,共5页China Journal of Chinese Materia Medica

基　　金：甘肃教育厅科研项目(0906B-07);甘肃中医学院中青年科研基金项目(09ZQ-17;1106B-02)

摘　　要：目的:优选大孔吸附树脂纯化铁棒锤总生物碱的最佳条件,比较不同产地铁棒锤中总生物碱与乌头碱的含量,为进一步研究铁棒锤质量提供依据。方法:采用正交设计优选大孔吸附树脂对铁棒锤总生物碱的最佳纯化条件,以滴定法测定总生物碱的含量。采用最佳工艺条件对4个不同产地铁棒锤的总生物碱进行纯化,并用HPLC测定乌头碱的含量。结果:不同工艺条件对总生物碱的纯化有不同影响。最佳纯化条件为树脂型号HPD-722,80%乙醇,乙醇洗脱量80mL·min-1。HPLC测得青海、马衔山、宁夏、永登4个不同产地铁棒锤中乌头碱质量分数分别为0.4935,0.8835,1.5278,1.6644mg·g-1。结论:该文的最佳工艺条件可用于总生物碱和乌头类生物碱的纯化。不同产地铁棒锤中乌头碱含量以甘肃永登栽培最高。To optimize the conditions of purifying the total alkaloids in Aconitum szechenyianum with macroporous adsorption resin, and compare the content of total alkaloids and aconitine in A. szechenyianum from different producing areas, in order to provide basis for further studies. Method ： The orthogonal experiment method was adopted for optimizing the conditions of purifying the total alkaloids in A. szechenyianum with macroporous adsorption resin. The content of total alkaloids and aconitine were determined by using the titration method. The total alkaloids in A. szechenyianum from different producing areas were purified under optimum pro- cessing conditions. Aconitine was determined by HPLC. Result： Different processing conditions showed different influences on the purification of total alkaloids. The optimum conditions were resin type HPD-722, ethanol concentration of 80%, and ethanol elution volume of 80 mL · min- 1. The contents of aconitine in A. szechenyianum from different producing areas Qinghai, Maxianshan, Ningxia and Yongdeng were 0. 493 5, 0. 883 5, 1. 527 8, 1. 664 4 mg · g-1, respectively. Conclusion： The optimum processing conditions used in this essay could be used for purifying the total alkaloids and aconitine. A. szechenyianum from Yongdeng and Gansu contains the highest content of aconitine.

关 键 词：大孔吸附树脂 铁棒锤 总生物碱 乌头碱 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]