三种检测阴沟肠杆菌AmpC酶方法的比较  

Comparison of Three Methods for Detecting AmpC Enzymes Produced by Enterobacter Cloacae

作　　者：朱建峰[1] 张雪松[2] 

机构地区：[1]江苏省如东县人民医院检验科,226400 [2]江苏省如东县中医院检验科

出　　处：《医学理论与实践》2013年第8期1004-1005,1009,共3页The Journal of Medical Theory and Practice

摘　　要：目的:以三维实验结果为标准,比较表型筛选法和双抑制剂扩散协同法试验检测产AmpC酶阴沟肠杆菌的可靠性。方法:收集2011年2月-2012年12月我院临床分离的阴沟肠杆菌39例,分别用三维实验、表型筛选法、双抑制剂扩散协同法试验检测产AmpC酶阴沟肠杆菌。结果:39株阴沟肠杆菌经三维试验检测出高产AmpC酶的阴沟肠杆菌10株,检出率为25.6%。表型筛选法特异性为69.0%,灵敏度为90.0%,检出效率为76.9%。双纸片协同试验特异性为93.1%,灵敏度为80.0%,检出效率为89.7%。结论:双抑制剂扩散协同法具有较高的灵敏度和特异性,且与三维试验阳性检出率相当,是一种可靠的方法。Objective： To compare the reliability of phenotypic screening method and dual inhibitors diffuse synergy test in detecting AmpC enzymes produced by Enterobacter cloacae based on the results of 3D experiment. Methods： Collecting 39 cases who clinically isolated Enterobacter cloacae in our hospital from February 2011 to December 2012, detecting AmpC enzymes produced by Enterobacter cloacae with three-dimensional experiment, phenotypie screening method and dual inhibitors diffuse synergy test, respectively. Results： 10 cases highly produced AmpC enzymes in 39 Enterobacter cloacae by three-dimensional experiment, the detection rate was 25. 6%. The specificity, sensitivity and detection efficiency for phenotypic screening method were 69. 0%, 90. 0%, 76. 9%, respectively. And that for double disk synergy test were 93. 1%, 80. 0%,89. 7 %, respectively. Conclusion： Dual inhibitors diffuse synergy test is a reliable method for its high sensitivity and specificity, and it is consistent with the positive detection rate of three-dimensional test.

关 键 词：AMPC酶 三维试验 双纸片协同试验 表型筛选法 

分 类 号：R446[医药卫生—诊断学]