LC-MS/MS法测定生脉注射液中5种主要药效成分的含量  

Determination of Five Active Constituents in Shengmai Injection by LC-MS/MS

作　　者：邵玉凤[1] 王文艳[1] 林青雯[1] 马莎莎[1] 刘万卉[1] 

机构地区：[1]烟台大学药学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2013年第2期115-118,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家重点基础研究发展计划(973计划)前期研究专项基金资助项目(2012CB724003);"泰山学者"建设工程专项资助项目

摘　　要：建立了LC-MS/MS的方法同时测定生脉注射液中人参皂苷Rg1、Re、Rb1、Rd和五味子醇甲的含量.采用Lichrospher C18(150×2.1mm,i.d.,5μm)色谱柱;以乙腈-0.1%甲酸溶液(65∶35)为流动相;流速为0.2 mL·min-1,柱温为30℃.结果表明,在本研究建立的实验条件下人参皂苷Rb1、Rd、Re、Rg1和五味子醇甲5种成分在各自线性范围内的线性关系良好,精密度、重复性RSD均小于3%.本方法灵敏度高、简便快捷,可同时快速测定生脉注射液中的5种成分的含量.A liquid chromatography-tandem mass spectrometric method is established for simultaneous determination of ginsenoside Rbl ,Rd,Re,Rgl and sehizadrin in Shengmai Injection. The analytical column is a Lichrospher C18 （150 ×2. 1 mm, i. d. , 5 μm）. The mobile phase consists of acatonitril-0. 1% formic acid solution （65： 35, V/V） at a flow rate of 0.2 mL· min^- 1 The sensitivity of ginsenoside Rgl, Re, Rbl, Rd and schizadrin are favorable in this Chromatographic conditions. All the compounds show good linearity in their corresponding detection range. The RSD of precision, repeatability and recovery in experiments are less than 3%. This method is accurate, simple, reliable and reproducible to determine the 5 active constituents in the Shengmai Injection.

关 键 词：生脉注射液 人参皂苷 五味子醇甲 HPLC—MS MS 

分 类 号：R917[医药卫生—药物分析学]