α1受体拮抗剂对糖尿病鼠肾组织Ⅳ型胶原及Smad2／3表达的影响  

Effects of Cardura on collagen Ⅳ and Smad2/3 expression in autoantibodies against α1-adrenergic receptot-mediated diabetic rats

作　　者：白伟伟[1] 赵林双[1] 谭学莹[1] 

机构地区：[1]广州军区武汉总医院内分泌科,广州430070

出　　处：《临床肾脏病杂志》2013年第3期133-136,共4页Journal Of Clinical Nephrology

基　　金：湖北省卫生厅资助项目,JX5C18

摘　　要：目的探讨α1受体拮抗剂多沙唑嗪对糖尿病大鼠的肾脏保护作用。方法动物分为正常对照组、单纯糖尿病组、糖尿病鼠抗α1受体抗体介导组、糖尿病鼠抗α1受体抗体介导并多沙唑嗪干预组和糖尿病鼠无抗α1受体抗体介导多沙唑嗪干预组，腹腔注射链脲佐菌素法建立糖尿病大鼠模型，尾静脉注射抗α1受体抗体的方法进行介导，干预组给予多沙唑嗪3．6mg·kg^-1·d^-1。观察肾脏超微病理改变。免疫组化法检测肾组织Ⅳ型胶原和Smad2／3。结果糖尿病鼠抗α1受体抗体介导组Ⅳ型胶原和Smad2／3蛋白的表达较单纯糖尿病组明显增多（均P＜0．05）。糖尿病鼠抗α1受体抗体介导多沙唑嗪干预组与糖尿病鼠无抗α1受体抗体介导多沙唑嗪干预组比较，肾皮质中Ⅳ型胶原和Smad2／3蛋白的表达较低（均P〈O．05）。结论多沙唑嗪对糖尿病大鼠肾脏病变具有保护作用。Objective To investigate the protective effects of on collagen Ⅳ and Smad2/3 expression in autoantibodies against α1-adrenergic receptor （α1-AA）-mediated diabetic rats. Methods Fifty-six male Wistar rats of 8 weeks age were randomly divided into a normal control group （n = 8） and a model group （n = 48）. Rats in the normal control group were fed of regular diet, while those in the model group were fed on high-fat high-sugar diet, and 4 weeks later given intraperitoneal injection of 30 mg/kg streptozotocin（STZ）. The successfully constructed type 2 diabetic nephropathy rat models were then randomly divided into DM group （n = 11 ）, DM ＋α1-AA group （n = 12）, DM ＋α1-AA ＋ group （n = 12） and DM ＋ group （n = 12）. Rats in al-AA group were given intraperitoneal injection of 100 μg/100g α1-AA every 4 weeks, was administered with 3.6 mg·kg^-1. day^-1. The ultrasturctural pathological changes of the kidney were examined. Renal tissue collagen Ⅳand Smad2/3 expression was detected by using immunohistochemistry. Results The expression of collagen Ⅳ and Smad2/3 expressions in DKD group and α1-AA group was significantly higher than in normal control group （P〈0. 05）. Both collagen Ⅳ and Smad2/3 expression levels in group were lower than in DKD group and α1- AA group （P〈0. 05）. Conclusions Treatment could mildly protect kidneys of diabetic rats by inhibiting the collagen Ⅳ and Smad2/3 expression.

关 键 词：抗体 糖尿病 大鼠 

分 类 号：R972.4[医药卫生—药品]