基于新型共反应物的流动注射电化学发光分析方法研究  

Study of the Flow Injection Electrochemiluminescence Analysis System Based on a New Co-reactant

作　　者：陈然[1,2] 王捷[2] 刘仲明[2] 陈钰[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]广州军区广州总医院医学实验科,广东广州510010

出　　处：《分析科学学报》2013年第2期219-222,共4页Journal of Analytical Science

基　　金：广东省省部产学研项目(No.2010B090400187);广东省科技计划(No.2009B030801280)

摘　　要：为提高钌联吡啶电化学发光强度,对比研究了低浓度Ru(bpy)23+与N-丁基二乙醇胺、N,N-二丁基乙醇胺和三丙胺三种常用胺类共反应物的电化学发光响应情况,结果表明Ru(bpy)23+/N-丁基二乙醇胺体系的电化学发光特性最佳。实验对该体系在流动注射电化学发光分析系统中的检测条件进行了优化。在电位1.8V,进样量150μL,推速30μL/s的最优检测条件下,获得Ru(bpy)23+的检出限(S/N=3)为5.0×10-10 mol/L。方法线性范围为1.0×10-9～5.0×10-7 mol/L;重复性良好,相对标准偏差(RSD)<5%。To improve the electrochemiluminescence（ECL） of Ru（bpy）23＋, a comparison of the eiticlency of three common effective co-reactants such as N-butyldiethanolamine（BDEA）,2-（dibutylamino）ethanol and triethylamine at low Ru（bpy）23＋ concentration has been reported. N-butyldiethanolamine was found to be the best co-reactant and showed strong ECL efficiency,then the optimal detection conditions of the Ru（bpy）23＋/BDEA system was studied. Under the optimal conditions such as detection potential at 1.8 V and sample injection of 150 μL at 30 μL/s, the ECL intensity was linear with the concentrations of Ru（hpy）g＋ in the range of 1.0×10-9-5.0×10-7 mol/L with a detection limit（S/N=3） of 5.0X 10-10 mol/L.

关 键 词：钌联吡啶 共反应物 电化学发光 流动注射 

分 类 号：O657.39[理学—分析化学]