辛伐他汀对人正常牙髓细胞和脂多糖诱导的人牙髓细胞增殖的影响  

Effects of simvastatin on the proliferation of human dental pulp cells and lipopolysaccharide-induced human dental pulp cells

作　　者：关为群[1] 陈雅瑜[2] 

机构地区：[1]福建医科大学附属协和医院,福建福州350001 [2]福建医科大学口腔医学院,福建福州350004

出　　处：《中国医院药学杂志》2013年第7期522-526,共5页Chinese Journal of Hospital Pharmacy

基　　金：福建省卫生厅创新课题(编号:2011-CXB-6)

摘　　要：目的:探讨不同浓度辛伐他汀对体外培养人正常牙髓细胞增殖及LPS诱导的人牙髓细胞的影响,为寻求最佳影响浓度提供参考依据。方法:以改良组织块法体外获得牙髓细胞,选择不同浓度级别的辛伐他汀4组(0.001,0.01,0.1,1μmol·L-1)作用于细胞,与空白对照组相比,观察能促进人正常牙髓细胞增殖的浓度;进一步细分同一级别的辛伐他汀浓度范围(0.005,0.01,0.015,0.05μmol·L-1)作用于人正常牙髓细胞及脂多糖诱导的人牙髓细胞。结果:高浓度(1μmol·L-1)辛伐他汀抑制人正常牙髓细胞增殖,与对照组相比有统计学意义。而0.01μmol·L-1组辛伐他汀促进人正常牙髓细胞增殖,与对照组相比有统计学意义。从第1天起,0.005,0.01,0.015μmol·L-1及0.05μmol·L-1组辛伐他汀抑制脂多糖诱导的人牙髓细胞增殖;而0.01μmol·L-1抑制效果最明显。结论:辛伐他汀对人牙髓细胞增殖的影响与其浓度有关。高浓度1μmol·L-1则抑制人正常牙髓细胞增殖,0.01μmol·L-1辛伐他汀促进人正常牙髓细胞增殖,而抑制脂多糖诱导的人牙髓细胞增殖。ONIECTIVE To investigate the effects of different concentrations of simvastatin on the proliferation of human dental pulp cells and LPS-induced human dental pulp cells in vitro,to find out the most effective concentration of simvastatin.METHODS Human dental pulp cells were obtained from modified cultured tissues. The pulp cells were divided into 5 groups randomly, simvastatins were added into each group until the ultimately concentration were （0.001,0.01,0.1,1μmol·L^-1 respectively while the group without simvastatin as control group. Then, the suitable concentrations of simvastatin on the proliferation of human dental pulp cells were observed. The former concentrations of simvastatin were further subdivided into 0. 005,0. 01,0. 015,0. 05 μmol·L^-1 added into the human dental pulp cells and LPS-induced human dental pulp ceils. IIESULTS The high- est concentration of simvastatin was μmol·L^-1 which inhibited the cell proliferation. There was significant difference between experimental group and control group. 0.01μmol·L^-1 simvastatin significantly enhanced cell proliferation of human dental pulp cells, and there was significant difference compared with control group.0. 005,0. 01 ,0. 015,0. 05 μmol·L^-1 were all inhibited the proliferation of LPS-induced dental pull cells, and the most significant concentration was 0. 01 μmol·L^-1. CONCLUSION The effect of simvastatin on proliferation of human dental cells was related with its concentration. High concentration （1 μmol·L^-1）inhibited human dental pulp cells＇ proliferation, but low concentration （0. 01μmol·L^-1） enhanced human dental pulp cellg proliferation and inhibited the proliferation of LPS-induced human dental pulp cells.

关 键 词：辛伐他汀 人牙髓细胞 脂多糖 CCK-8 增殖 

分 类 号：R966[医药卫生—药理学]