脑肠肽Ghrelin对脓毒症大鼠小肠上皮短肽载体1调节作用的研究  

Effect of Ghrelin on PepT1 of the small intestinal epithelium in rats with sepsis

作　　者：刘景全[1] 石斌[2] 马国光[1] 娄晓丽[3] 刘鸿翔[2] 梁冬雨[3] 万晟霞[1] 

机构地区：[1]兰州大学第一临床医学院重症医学科,甘肃730001 [2]上海市松江区中心医院急诊危重病科,201600 [3]上海市松江区中心医院中心实验室,201600

出　　处：《中华危重病急救医学》2013年第4期233-237,共5页Chinese Critical Care Medicine

基　　金：上海市卫生局科研项目（20124307）

摘　　要：目的探讨脑肠肽Ghrelin对脓毒症大鼠小肠上皮短肽载体1（PepT1）表达及功能的影响。方法雄性SD大鼠80只，按随机数字表法分为正常组、假手术组、脓毒症组和Ghrelin干预组4组，每组20只。采用盲肠结扎穿孔术（CLP）制备脓毒症大鼠模型；制模后即静脉给予Ghrelin干预。各组术后20h随机取10只大鼠，进行小肠黏膜病理观察，实时定量聚合酶链反应（PCR）和蛋白质免疫印迹试验分别检测PepT1的mRNA和蛋白表达，同时检测小肠上皮PepT1对底物Gly—Sar的摄取浓度；另10只大鼠记录7d生存率。结果与正常组和假手术组相比，脓毒症组小肠黏膜受损明显，PepT1mRNA表达（21．43±1．37比23．29±1．24、23．23±1．48）、蛋白表达（7832．55±699．74比13584．74±360．66、13152．51±605．72）以及Gly—Sat浓度（60min：3．23±0．30比6．67±0．51、6．52±0．47）均明显降低（均P〈0．05）；与脓毒症组比较，Ghrelin干预组大鼠小肠黏膜损伤较轻，生存率（40％比20％）、PepT1mRNA表达（22．73±1．16比21．43±1．37）、蛋白表达（10506．26±850．59比7832．55±699．74）及Gly—Sar浓度（60min：4．83±0．32比3．23±0．30）均显著升高（均P〈0．05）。正常组与假手术组各指标比较无显著差异。结论脓毒症大鼠小肠上皮PepTlmRNA及蛋白表达明显下降，机体在基因及蛋白水平下调了小肠上皮PepT1生物学功能；Ghrelin干预对脓毒症小肠上皮PepT1mRNA、蛋白表达以及摄取能力均有上调作用。Objective To investigate the effect of Ghrelin on expression and function of PepT1 of the small intestinal epithelium in rats with sepsis. Methods Eighty male Sprague-Dawley （SD） rats were divided into four groups by random number table ： normal group, sham operation group, sepsis group and Ghrelin group, with 20 rats in each group. The model of sepsis was reproduced with cecal ligation and puncture （CLP）. After the procedure Ghrelin was injected via vein in the Ghrelin group. Ten rats in each group were used to study the intestinal pathology. In addition, real time polymerase chain reaction （PCR） and Western blotting were used to detect PepT1 mRNA expression and PepT1 protein expression levels respectively in each group. The uptake of PepT1 by small intestinal epithelial ceils was also measured. Seven-day survival was observed in other 10 rats of groups. Results Compared with normal and sham operation groups, the damage to the small intestine mucosa was more serious in sepsis group, and the PepT1 mRNA expression （21.43 ± 1.37 vs. 23.29 ± 1.24, 23.23 ± 1.48）, the PepT1 protein expression （7832.55 ± 699.74 vs. 13 584.74 ± 360.66, 13 152.51 ± 605.72） and the uptake of PepT1 （60 minutes： 3.23 ± 0.30 vs. 6.67 ± 0.51, 6.52 ± 0.47 ） in the sepsis group were significantly lowered （all P〈0.05 ）. Compared with the sepsis group, less damage to the small intestine mueosa was found in the Ghrelin group, and the survival rate of rats （40% vs. 20% ）, PepT1 mRNA expression （22.73 ± 1.16 vs. 21.43 ± 1.37）, PepT1 protein expression （ 10 506.26 ± 850.59 vs. 7832.55 ± 699.74） and the uptake of PepT1 （60 minutes： 4.83 ± 0.32 vs. 3.23 ± 0.30 ） were significantly improved in the Ghrelin group （all P〈 0.05 ）. There was no difference in various indicators between sham operation and normal groups. Conclusions The PepT1 mRNA and protein expression of the small intestinal epithelium in septic rats were significantly decreased, which affected the physiological function

关 键 词：脓毒症 GHRELIN 短肽载体1 盲肠结扎穿孔术 

分 类 号：R45[医药卫生—治疗学]