一种鉴定4种根结线虫的PCR方法  

An Improved Polymerase Chain Reaction Method for Identification of Four Major Root Knot Nematodes

作　　者：景晓辉[1] 吴伦英[1] 汪军[2] 黄俊生[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室海南省热带作物病虫害生物防治工程技术研究中心,海南海口571101

出　　处：《热带作物学报》2013年第3期433-435,共3页Chinese Journal of Tropical Crops

基　　金：国家公益性行业(农业)科研专项(No.200903049);海南省研究生创新科研课题(No.Hyb2012-1)

摘　　要：根结线虫(Meloidogyne spp.)是威胁全球农业生产的重要病原物,每年给农业生产造成巨大的经济损失。利用两对引物#C2F3/#1108和MI-F/MI-R建立了一种适用于4种根结线虫分子鉴定的技术。结果表明:引物#C2F3/#1108可将根结线虫分为南方根结线虫或爪哇根结线虫、花生根结线虫和北方根结线虫;引物MI-F/MI-R可用于特异性区分南方根结线虫和爪哇根结线虫。A rapid and sensitive polymerase chain reaction （PCR） method for the detection and identification of four most common and economically important Meloidogyne spp. was developed. By using previously published primer #C2F3/#1108, fragments of three sizes were detected. The M. incognita and M. javanica reactions produced a 1.7 kb fragment; the M. arenaria reaction, a 1.1 kb fragment; and the M. hapla reaction resulted in a 0.5 kb fragment. Based on the sequence of oesophageal gland protein gene SEC-1, specific primer MI-F/MI-R was designed and tested for its amplification specificity and efficiency against populations of M. incognita. This resulted in two pairs of primers that were used in combination to reliably identify Meloidogyne incognita, M. arenaria, M. javanica and M. hopla.

关 键 词：聚合酶链式反应 食管腺蛋白基因 根结线虫 鉴定 

分 类 号：S432[农业科学—植物病理学]