金弹试管苗叶片MLP2基因的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of MLP2 Gene from the Leaves of in vitro Plantlets of Fortunella crassifolia

作　　者：金建涛[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《热带作物学报》2013年第3期442-450,共9页Chinese Journal of Tropical Crops

基　　金：福建省重大科技平台建设项目(No.2008N2001);国家支撑计划资助项目(No.2007BAD07B01)

摘　　要：采用RT-PCR结合RACE技术,以金弹(尤溪金柑Fortunella crassifolia cv.Youxijingan)试管苗的叶片为材料,克隆得到金弹Fc-MLP2基因的全长cDNA序列,其在GenBank中的登录号为JX310276。Fc-MLP2基因全长908 bp,含有一个669 bp的开放阅读框,编码223个氨基酸。生物信息学分析表明:该蛋白是定位于细胞外的一种疏水性分泌蛋白,具有2个功能位点、13个磷酸化位点和信号肽,其二级结构由α螺旋、β折叠、β转角、无规则卷曲组成。系统进化树分析表明,金弹Fc-MLP2与粗皮柠檬的RlemMLP2蛋白亲缘关系较近,聚为同一类。该蛋白可能在植物抗虫、抗病原菌、抗饥饿和延缓植物衰老方面起到一定作用。The Fc-MLP2 cDNA sequence was cloned from the leaves of Fortunella crassifolia cv. Youxijiugan using RT-PCR and RACE techniques. The accession number was JX310276 in GenBank. The cDNA sequence consisted of 908 bp with an intact open reading frame of 669 bp, encoding a polypeptide of 223 amino acids. Bioinformatics analysis showed that the protein was a hydrophobic secretory protein located in extracellular position, with two functional sites, 13 phosphorylation sites and a signal peptide. The secondary structure was made of alpha helixes, beta sheets, beta turns and coils. Phylogenetic analysis also indicated that the Fc-MLP2 was close to RlemMLP2 of Citrus jambhiri in genetic relationship.

关 键 词：金弹 试管苗 Fc—MLP2 基因克隆 生物信息学 

分 类 号：Q344.14[生物学—遗传学]