双向电泳-质谱技术寻找原发性高尿酸血症血清差异蛋白初探  

Study of differentially expressed serum proteins in primary hyperuricemia by two-dimentional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry

作　　者：于飞[1] 赵建梅[1] 陈兆云[1] 李巍[1] 姚华[2] 

机构地区：[1]新疆医科大学第一附属医院医学检验中心,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院党办

出　　处：《中国慢性病预防与控制》2013年第2期143-145,149,共4页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：新疆维吾尔自治区自然科学基金青年基金(2012211B33)

摘　　要：目的通过对比分析正常人和高尿酸血症患者血清蛋白双向电泳图谱,初步分析高尿酸血症血清差异蛋白。方法采用双向凝胶电泳(2-dimentional gel electrophoresis,2-DE)和基质辅助激光解吸飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry,MALDI-TOF-MS)对高尿酸血症患者和对照组健康人血清进行分析,寻找高尿酸血症血清差异蛋白。结果差异表达蛋白质点数为10个,质谱成功鉴定出4个差异蛋白质,其中补体C3、触珠蛋白、α1-抗胰蛋白酶呈上调表达,载脂蛋白L1呈下调表达。结论补体C3、触珠蛋白、α1-抗胰蛋白酶、载脂蛋白L1在高尿酸血症组和正常对照组血清中存在差异,但结果有待运用其他生物学的方法进一步验证。Objective To study the differentially expressed serum proteins between controls and patients with primary hyperuricemia （HUA） by two-dimensional gel electrophoresis （2-DE）. Methods 2-DE and matrix-assisted laser desorption/ ionization time-of-flight tandem mass spectrometry（ MALDI-TOF-MS ） were used to detect the serum of controls and patients with HUA for screening the differentially expressed serum proteins. Results There were 10 differentially expressed serum protein spots by gel-image analysis. Four differentially expressed serum proteins were successfully identified with MALDI-TOF-MS. The complement 3, haptoglobin and otl-antitrypsin were up-regulated and apolipoprotein L1 was down-regulated in patients with HUA, as compared with controls. Conclusion The complement 3, haptoglobin, α1-antitrypsin and apolipoprotein L1 are differentially expressed between controls and patients with HUA and the result should be validated by other biochemical technologies.

关 键 词：高尿酸血症 血清 蛋白质组学 双向电泳 

分 类 号：R589[医药卫生—内分泌]