荧光增白剂与增效蛋白对美国白蛾核型多角体病毒(HcNPV)的增效作用  

Enhancing effects of Fluorescent Brightener 28 and Helicoverpa armigera granulovirus enhancin on Hyphantria cunea nucleopolyhedrovirus

作　　者：刘海明[1] 周洪旭[1] 李长友[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东省植物病虫害综合防控重点实验室,山东青岛266109

出　　处：《农药学学报》2013年第2期153-158,共6页Chinese Journal of Pesticide Science

基　　金：山东省教育厅项目(J10LC07);青岛农业大学博士基金(6631024);“‘泰山学者’建设工程专项经费”资助

摘　　要：围食膜是昆虫中肠细胞与肠腔之间的一种防御性屏障,破坏围食膜即能促进病原微生物对昆虫的侵染。分别研究了荧光增白剂FB28和棉铃虫Helicoverpa armigera颗粒体病毒增效蛋白(En-Ha)对美国白蛾核型多角体病毒(Hyphantria cunea nucleopolyhedrovirus,HcNPV)的增效作用,分析了活体条件下FB28与En-Ha对美国白蛾幼虫围食膜的破坏作用,解释了其增效作用机理。生物测定结果表明,FB28和En-Ha均能够影响围食膜蛋白的表达谱,提高HcNPV的感染率,使其毒力显著提高:单独饲喂HcNPV时的LC50值为1.11×105PIBs/mL,添加FB28和En-Ha后,其LC50值分别降低为1.91×103和7.70×103PIBs/mL,增效比值(SR)分别为58.12和14.42。SDS-PAGE电泳结果发现:用FB28饲喂处理幼虫后,分子质量为60 ku的蛋白条带消失,37 ku的新蛋白条带逐渐出现;幼虫摄取含有En-Ha的饲料后,分子质量为75 ku的蛋白条带消失,44.3 ku的新蛋白条带逐渐出现。经过10～24 h后,FB28和En-Ha处理的幼虫围食膜都能够恢复正常,表明其破坏作用是短暂的。The peritrophic membrane （PM） is an invertebrate-unique semi-permeable structure secreted from midgut epitheliums. As a barrier to infection and mechanical damage, it plays an important role in protecting the midgut epithelium from pathogens, toxins, and other damaging factors. The disruptive effects of Fluorescent Brightener 28 （ FB28 ） and Helicoverpa armigera granulovirus enhancin （En-Ha） on the peritrophic membrane of Hyphantria cunea larvae as well as their enhancing effects on the toxicity of H. cunea nucleopolyhedrovirus （HcNPV） were studied. The result of bioassay showed that FB28 and En-Ha could disrupt the structure of PM and enhance the vitality of HcNPV. The LC50 value decreased to 1.91 ×10^3 and 7.70 × 10^3 PlBsJmL, and the synergism rate were 58. 12 and 14.42, respectively, after feeding diets containing FB28 ＋ HcNPV and En-Ha ＋ HcNPV. The results of SDS- PAGE indicated that after the larvae were reared with diets containing FB28, the protein with molecular weight 60 ku disappeared and some with molecular weight 37 ku appeared;after the larvae were reared with diets containing En-Ha, the protein with molecular weight 75 ku disappeared and some with molecular weight 44.3 ku appeared. However, the disappeared protein bands recovered again after 10 - 24 hours, suggesting the effects of FB28 and En-Ha on PMs of H. cunea was temporary and reversible.

关 键 词：荧光增白剂 增效蛋白 美国白蛾 围食膜 核型多角体病毒 

分 类 号：S482.91[农业科学—农药学]