麦田抗性生物型荠菜对苯磺隆的抗性机制研究  

Mechanism of resistance to tribenuron-methyl in a resistant biotype of Capsella bursa-pastoris from wheat fields

作　　者：毕亚玲[1,2] 刘君良[1] 王兆振[1] 卜东欣[1] 郭文磊[1] 王金信[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]安徽科技学院植物科学学院,安徽凤阳233100

出　　处：《农药学学报》2013年第2期171-177,共7页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金面上项目(31171866);国家自然科学基金青年科学基金(31201529);公益性行业(农业)科研专项(201303031)资助

摘　　要：为明确抗性生物型荠菜对苯磺隆的抗性机制,分别测定了苯磺隆对抗性和敏感生物型荠菜体内乙酰乳酸合成酶(ALS)、谷胱甘肽-S-转移酶(GSTs)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的影响。结果表明:离体条件下,抗性生物型荠菜体内ALS对苯磺隆的敏感性明显降低,苯磺隆对荠菜抗性和敏感生物型ALS的抑制中浓度(I50)分别为0.722 8和0.052 1μmol/L,抗性与敏感生物型I50的比值为13.87;活体条件下,施用苯磺隆后,抗性和敏感生物型荠菜ALS活性均受到一定程度的抑制,但抗性生物型ALS活性受到抑制后能逐渐恢复,而敏感生物型则不能恢复;经苯磺隆处理后,抗性生物型GSTs相对活力明显高于敏感生物型,而抗性和敏感生物型体内POD、SOD和CAT相对活力无明显差异。研究表明,抗性生物型荠菜体内ALS对苯磺隆敏感性降低是其抗药性产生的原因之一,而GSTs对苯磺隆代谢能力的差异也可能与荠菜对苯磺隆的抗性有关。To ascertain the resistance mechanism of Capsella bursa-pastoris to tribenuron-methyl, the enzyme assays of ALS, GSTs, POD, SOD and CAT in both resistant （R） and susceptible （S） biotypes of C. bursa-pastoris were conducted. The results of in vitro test showed that the sensitivity of ALS to tribenuron-methyl was decreased in the R biotype, the Wibenuron-methyl concentration causing 50% inhibition of ALS activity （ I50 ） in R and S biotypes was 0. 722 8 and 0.052 1 μ mol/L, respectively, and the relative resistance index [I50 （R）/I50 （S）] was 13. 87. The results of in vivo experiment indicated that ALS activities were inhibited in both R and S biotypes. The ALS activity of the R biotype could be recovered, while that of the S biotype could not; the GSTs activity of R biotype was obviously higher than that of S biotype; the activities of POD, SOD and CAT in R and S biotypes showed no obvious difference. The results suggested that reduced sensitivity of ALS was one of the resistance mechanisms to tribenuron-methyl in C. buusa-pastoris, and the metabolic ability of GSTs might be also related with the resistance.

关 键 词：荠菜 苯磺隆 抗药性 乙酰乳酸合成酶 

分 类 号：S481.4[农业科学—农药学]