13C红外光谱仪测定呼气中13C千分差值的可信度研究  

Reliability study on the infrared spectrometry for measuring the delta over baseline for breath ^(13)C

作　　者：王敏[1] 王志玲[2] 勾凌燕[2] 张宇辉[2] 杨晓光[3] 沙磊[1] 李敏[3] 

机构地区：[1]中国疾病预防控制中心,北京102206 [2]中国人民解放军白求恩军医学院 [3]中国疾病预防控制中心营养与食品安全所

出　　处：《卫生研究》2013年第2期298-301,共4页Journal of Hygiene Research

基　　金：国家自然科学基金青年科学基金(No.81001247)

摘　　要：目的评估13C红外光谱仪测定呼气中13C千分差值的可靠性。方法选取健康成年人24名,男女各半。以13C-亮氨酸作为示踪剂进行稳定性同位素代谢实验,收集服用稳定性同位素前基础呼气,并于服用同位素后收集60、120、180、195、210、225、240、255、270、300 min呼气,采用13C红外光谱仪和Heliview1 3C-呼气分析仪测定呼气中13C千分差值。结果采用两因素方差分析得到两种测量仪器测试结果F=0.29,P=0.5874;一致性检验结果 t=0.48,P=0.6346。两种测量仪器测定呼气中13C千分差值无显著性差异统计,两种测量方法较为一致。结论13C红外光谱仪测定呼气中13C千分差值的结果可靠。Objective To assess the validity and reliability of the infrared spectrometry for measuring the delta over baseline for breath 13 C. Method Twenty-four healthy adults were selected, including twelve males and twelve females. 13C-Leucine was used as tracer in stable isotope metabolic experiments. One baseline breath was collected before the tracer protocol began. Other breath samples were collected at 60, 120, 180, 195, 210, 225, 240, 255, 270 and 300 min post-tracer challenge. The delta over baseline for breath 13C was measured by the infrared spectrometry and Heliview 13C breath analyzer. Results No significant differences were found between the infrared spectrometry and Heliview 13C breath analyzer in measuring the delta over baseline for breath 13C. The F value and p value of two-way ANOVA were 0.29 and 0.5874 respectively. The t and p value of consistency test were 0.48 and 0. 6346 respectively. Conclusion The 13C infrared spectrometry can reliably measure the delta over baseline for breath 13 C.

关 键 词：13C呼气实验 红外光谱法 质谱法 

分 类 号：R446[医药卫生—诊断学] TH744.41[医药卫生—临床医学]