头针对脑缺血再灌注大鼠神经干细胞增殖分化干预效果的实验研究  

Effect of Scalp-Acupuncture on Cerebral Ischemia/Reperfusion Rats of Proliferation and Differentiation Intervention Neural Stem Cell

作　　者：乐薇[1] 刘银妮[2] 王琼[1] 张红星[2] 黄国付[2] 周利[2] 

机构地区：[1]湖北中医药大学 [2]武汉市中西医结合医院,湖北武汉430022

出　　处：《湖北中医药大学学报》2013年第2期12-15,共4页Journal of Hubei University of Chinese Medicine

基　　金：湖北省自然科学基金项目(2006ABA326)

摘　　要：目的观察大鼠脑缺血再灌注损伤后反应性星形胶质细胞及神经细胞的活化增殖情况,探讨头针治疗脑缺血的可能机制。方法将Wistar雌性大鼠随机分为假手术组、模型组、头针组(模型组和头针组再根据缺血再灌注时间的不同,随机分为7d、14d、28d共6个亚组)。采用大脑中动脉线栓法制备大脑中动脉闭塞(MCAO)再灌注模型,选取顶颞后斜线、顶颞前斜线,进针后接韩氏穴位神经刺激仪。应用神经功能缺损评分(NSS)、免疫荧光双标方法观察各组缺血再灌注后不同时间点缺血侧室管膜下区及海马齿状回5-溴脱氧尿核苷(BrdU)、BrdU+胶质纤维酸性蛋白(GFAP)阳性细胞数值的变化。结果头针组与模型组各时相上的组间比较,第28天头针组的NSS显著低于模型组(P<0.01);与模型对照组比较,头针组于缺血再灌注第7天缺血侧室管膜下区、海马BrdU、BrdU+GFAP阳性细胞数基本相同,第14天、28天时均明显升高。结论大鼠缺血再灌注损伤后可引起其缺血侧室管膜下区、海马星形胶质细胞及神经细胞反应和增殖,而头针可显著增加大脑中动脉阻塞大鼠的神经干细胞增殖分化能力,实现对缺血脑组织保护的目的。Objective To observe cerebral ischemia rats of proliferation and differentiation in reactive astrocytes and neural cell.To explore the possible mechanisms of Scalp-acupuncture（SA） in treating cerebral ischemia.Methods Rat models with middle cerebral artery occlusion（MCAO） were used and 70 models were randomized into model group and SA group（inserting needles along anterior oblique line of vertex-temporal and posterior oblique line of vertex-temporal,soon after when a Han＇s Electro-Acupuncture Apparatus was connected,selecting ＂disperse-dense＂ wave,alternating frequency of 2 Hz and 100 Hz at an intensity level of 2 mA,20min per time,1 time each day） and applied neurological severity score（NSS）,double-label immunofluorescence assays in observing changes of Brdu、Brdu＋Glial fibrillary acidic protein positive cells in subventricular zone and dentate gyrus.Results（1） On NSS at each phase point： SA group demonstrated obvious decline in NSS at 28d when compared with that of model group（P0.01）.（2） By comparison,the number of SA group of Brdu、Brdu＋Glial fibrillary acidic protein positive cells in subventricular and dentate gyrus zone is the same as control group at 7d,but significantly higher at 14d and 28d.Conclusions The cerebral ischemia rats led to proliferation and differentiation of reactive astrocytes and neural cell in subventricular and dentate gyrus zone.By developing the proliferation and differentiation of Neural stem cell of MCAO rats,scalp-acupuncture is beneficial for protecting cerebral ischemia.

关 键 词：头针 神经干细胞 脑缺血再灌注 5-溴脱氧尿核苷 胶质纤维酸性蛋白质 

分 类 号：R245.9[医药卫生—针灸推拿学]