肺筋草化学成分研究  

Study on chemical constituents of Aletris spicata

作　　者：黄兰[1,2] 潘杰[1,2] 曹佩雪[2] 潘卫东[2] 梁光义[2,3] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [3]贵阳中医学院,贵州贵阳550002

出　　处：《中草药》2013年第7期812-815,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然基金项目(81160390);贵州省优秀青年科技人才培养计划项目(黔科合人字[2011]33号);贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2010]71号)

摘　　要：目的研究肺筋草Aletris spicata全草的化学成分。方法利用正、反相硅胶柱色谱、薄层色谱、Sephadex LH-20柱色谱及半制备高效液相色谱等方法进行分离纯化,然后根据理化性质和波谱数据鉴定化合物的结构,并对其中的三萜类化合物(化合物1～3)进行了抗肿瘤活和抗菌活性初步筛选。结果从肺筋草的干燥全草中分离得到了9个化合物,分别鉴定为24-甲基-9,19-环羊毛甾-24-烯-3-醇(1)、24-甲基-9,19-环羊毛甾-25-烯-3-醇(2)、24,24-二甲基-环木菠萝烷-3-醇(3)、美商陆酚A(4)、异美商陆酚A(5)、9′-methyl americanol A(6)、1-(4′-羟基苯基)-7-(3″-甲氧基-4″-羟基苯基)-4-烯-3-庚酮(7)、methyl-9,12,13-trihydroxyoctadeca-10E,15Z-dienoate(8)、5-羟甲基-2-呋喃甲醛(9)。结论所有化合物均为首次从该属植物中分离得到。化合物1～3无抗肿瘤和抗菌活性。Objective To study the chemical constituents ofAletris spicata. Methods The compounds were isolated and repeatedly purified by silica gel, TLC, and Sephadex LH-20 column chromatography as well as semi-Prep HPLC, and their structures were elucidated by means of spectral analysis and physicochemical properties. The antitumor and antibacterial activities of triterpenoids （compounds 1--3） were primarily screened. Results Nine compounds were obtained and identified as 24-methyl-9, 19-cyclolanost- 24-en-3-ol （1）, 24-methyl-9, 19-cyclolanost-25-en-3-ol （2）, cycloneolitsol （3）, americanol A （4）, isoamericanol A （5）, 9＇-methyl americanol A （6）, 1-（4＇-hydroxyphenyl）-7-（Y＇-methoxyl-4＂-hydroxyphenyl）-heptene-4-en-3-one （7）, methyl-9, 12, 13-trihydroxy- octadeca-10E, 15Z-dienoate （8）, and 5-hydroxymethyl-2-furancarboxaldehyde （9）. Conclusion All the compounds are isolated from the plants in Aletris L. for the first time. No anti-tumor and anti-bacterial activities are found in compounds 1--3.

关 键 词：肺筋草 24-甲基-9 19-环羊毛甾-24-烯-3-醇 24 24-二甲基-环木菠萝烷-3-醇 美商陆酚A 5-羟甲基-2-呋喃甲醛 

分 类 号：R284.1[医药卫生—中药学]