不同处理方法及影响因素对地黄中梓醇量的影响  

Effects of various treatments and influencing factors on catalpol in Rehmannia glutinosa

作　　者：张科[1] 郭建华[1] 田成旺[2] 张铁军[2] 

机构地区：[1]天津医科大学药学院,天津300070 [2]天津药物研究院,天津300193

出　　处：《中草药》2013年第7期896-899,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的分析不同贮藏方式、炮制方法、温度、pH值和酶对梓醇量的影响。方法采用HPLC法测定鲜地黄不同炮制品及不同贮藏条件下梓醇量的变化,并在不同温度、pH值及酶的条件下,放置一定时间后测定梓醇量。结果鲜地黄中梓醇量明显高于其炮制品,抽真空冷冻下梓醇量几乎不变,而梓醇受pH影响较大,强酸或强碱条件下均不稳定,并随着温度升高而加剧这种变化,同时易受β-葡萄糖苷酶的影响。结论梓醇对热稳定,对酸、碱及β-葡萄糖苷酶不稳定。Objective To study the effect of different storage methods, processing methods, temperatures, pH values, and enzymes on the content of catalpol in Rehmannia glutinosa. Methods To investigate the content change of catalpol in different processed R. glutinosa under the different storage conditions by HPLC. And the content of catalpol was determined after being stored under different temperatures, pH values, and enzymes. Results The content ofcatalpol in fresh R. glutinosa was significantly higher than that in the processed ones. The content of catalpol in frozen under vacuum was almost unchanged. The content of catalpol was obviously influenced by pH value, especially in strong acid and alkali, and the change was enhanced at higher temperature. Meanwhile, the content of catalpol was easily influenced by β-glucosidenzyme. Conelusion Catalpol is stable to high temperature, while unstable to acid, alkali or β-glucosidenzyme.

关 键 词：鲜地黄 PH值 炮制 梓醇 高效液相色谱 

分 类 号：R282.4[医药卫生—中药学]