应用圆二色光谱研究交变应力对烟草细胞膜蛋白结构的影响  

THE EFFECT OF ALTERNATIVE STRESS ON THE MEMBRANE PROTEIN CONFORMATIONS OF TOBACCO CELLS BY CIRCULAR DICHROISM

作　　者：蔡国友[1] 侯玉霞[1] 丁晓岚 李涛[3] 沈子威[4] 席葆树[1] 

机构地区：[1]清华大学工程力学系生物力学研究所,北京100084 [2]中国农业大学应用化学系 [3]中国科学院力学所 [4]清华大学生物科学与技术系,北京100084

出　　处：《光子学报》2000年第4期289-292,共4页Acta Photonica Sinica

基　　金：国家自然科学基金资助项目

摘　　要：本文用圆二色光谱 (CD)研究了交变应力对烟草细胞膜蛋白结构的影响 采用本实验室研制的强声波发生装置来产生交变应力场 ,研究了不同强度和频率的交变应力场作用后烟草细胞膜蛋白结构的变化 研究结果表明 :细胞膜蛋白结构变化与应力的频率和强度密切相关 ,一定频率和强度范围内的交变应力能使得烟草细胞膜蛋白的二级结构发生明显的变化 ,表现为α螺旋的增加和 β转角的减少 这反映了细胞膜蛋白和磷脂相互作用的增强和细胞膜流动性的增加 ,进而为细胞的生长和分裂提供有利的条件 从细胞和分子水平研究交变应力对植物生长。In this paper,the effect of alternative stress on cultured tobacco protoplasts was studied by Circular Dichroism spectra(CD).The field of alternative stress was generated through a strong sound field system set up in our lab.We found that the effects of stress on cells depend greatly on the intensity and frequency of stress.In a certain range of intensity and frequency,the stress make a significant changes on membrane protein conformations.It cause a increase in α helix and decrease in β turn.The membrane protein conformation changes denote the increased interaction between membrane protein and lipids and the increased fluidity of membrane,and thus make the cell to grow and divide faster and easier.The enhancement of cell membrane fluidity might be one of the mechanisms of the promotion of plant growth by alternative stress.

关 键 词：交变应力 膜蛋白结构 烟草细胞 圆二色光谱 

分 类 号：S572.01[农业科学—烟草工业]