IL-17A、NF-κB与PR在不明原因早期自然流产蜕膜组织中的表达及意义  

Expression and significance in uterine decidua of unexplained of IL - 17A, NF - κB and PR early spontaneous abortion patients

作　　者：秦晓静[1] 刘福民[2] 刘小云[2] 

机构地区：[1]徐州医学院研究生学院2010级,江苏徐州221004 [2]徐州医学院附属医院中心实验室,江苏徐州221002

出　　处：《徐州医学院学报》2013年第2期132-135,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的探讨白细胞介素17A（IL-17A）、核因子κB（NF-κB）、孕激素受体（PR）与自然流产的关系。方法随机选取30例早期自然流产患者作为流产组，30例正常早孕人工流产孕妇为对照组。酶联免疫吸附法（ELISA法）检测蜕膜组织中IL-17A、NF-κB与PR蛋白的表达；免疫组化法检测IL-17A、NF-κB与PR在蜕膜组织中的表达，对其进行定位、半定量分析，并对其之间的相互关系进行分析。结果①流产组蜕膜组织中IL-17A和NF-κB蛋白表达水平高于对照组，PR蛋白表达水平低于对照组，差异有统计学意义（P〈0．05）。②流产组胞质中IL-17A的表达高于对照组（P〈0．05）；流产组核内NF-κB的表达高于对照组（P〈0．05），胞质中NF-κB的表达低于对照组（P〈0．05）；流产组胞质中和核内PR的表达低于对照组（P〈0．05）。③胞质中IL-17A与胞核中NF-κB呈正相关（r=0．500，P〈0．05），与胞质中NF-κB呈负相关（r=-0．499，P〈0．05）；核内NF-κB与核内PR呈负相关（r=-0．513，P〈0．05），核内NF-κB和与胞质内PR呈负相关（r=-0．511，P〈0．05）。结论蜕膜组织中IL-17A表达增强可能与不明原因早期自然流产有关。蜕膜组织中IL-17A的活化可增加NF-κB表达，进而减少PR表达并减弱其功能，可能是其发病机制之一。Abstract： Objective To evaluate the expression of interleukin 17A （ IL - 17A）, nuclear factor κB （ NF - κB） and progesterone receptor （PR） in the decidual tissue of unexplained first trimester spontaneous abortion, and to analyze the mechanisms of the changes of IL - 17A, NF - κB and PR in the decidual leading to abortion. Methods 30 first tri- mester spontaneous abortion and 30 normal first trimester pregnant women were randomly devided as abortion group and control group. Expression of IL - 17A, NF - κB and PR protein in deciduas of abortion group and control group was de- teted by enzyme linked immunosorbent assay （ELISA）. Immunoflunistochemistry was employed on abortion group and control group to show the location, quantity and interaction of IL - 17A, NF - κB and PR. Results ① Expression of proteion of IL- 17A, NF- κB was significantly higher in decidual of abortion group compared with that of control group （ P 〈 0. 05 ）, and the PR proteion was significantly lower （ P 〈 0. 05 ）.② The quantities of intracytoplasma IL - 17A are higher in abortion group than control group（P 〈0.05 ） ; Intranuclear NF - κB was higher in abortion group than control group （P 〈0.05）, but intracytoplasma NF - κB was lower in abortion group than control group（P 〈0.05） ; Both the in- tracytoplasma PR and intranuclear PR were lower in study group than control group（ P 〈 0. 05, P 〈 0. 05 ）. ③Intracyto- plasma IL - 17A had a positive correlation with intranuclear NF - κB （ r =- 0. 500, P 〈 0.05 ） , and a negative correlation with intracytoplasma NF - κB （r = -0. 499, P 〈 0.05 ） ; intranuclear NF - κB had a negative correlation with intranuclear PR （ r = - 0. 513, P 〈 0.05 ）, and a negative correlation with intracytoplasma PR （ r = - 0. 511, P 〈 0.05 ）. Conclusion The upregulation of IL - 17A in decidual of abortion group may contribute to unexplained first trimester spontaneous a- bortion. The activation of IL - 17A may increase the expressi

关 键 词：白细胞介素17A 核因子ΚB 孕激素受体 早期自然流产 

分 类 号：R714[医药卫生—妇产科学]