辣椒素对大鼠肠系膜阻力血管的舒张作用及其机制  

Vasodilating effect of capsaicin on rat mesenteric artery and its mechanism

作　　者：陈强[1,2] 朱欢欢[1] 张媛媛[1] 张媛[1] 王利宏[1] 郑良荣[1] 

机构地区：[1]浙江大学医学院附属第一医院心内科,浙江杭州310003 [2]浙江大学医学院附属义乌医院心内科,浙江义乌322000

出　　处：《浙江大学学报（医学版）》2013年第2期177-183,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金项目(81170167);浙江省重大科技专项计划项目(2011C14027);浙江省中医药优秀青年人才基金计划(2011ZQ013)

摘　　要：目的:观测辣椒素(capsaicin,CAP)对大鼠肠系膜阻力血管的舒张作用,探讨其可能机制。方法:雄性Sprague-Dawley大鼠,取肠系膜动脉三级分支约2 mm长血管环,置于DMT 610M系统,记录张力变化。结果:CAP(10-9～10-5mol/L)对苯肾上腺素(PE)预收缩的内皮完整血管和去内皮血管产生浓度依赖性的舒张作用,其中内皮完整组的血管舒张作用比去内皮组更明显,两者相比CAP在10-7～10-5mol/L时,差异有统计学意义(P<0.01)。该舒张作用可被一氧化氮合酶抑制剂L-NAME和CGRP竞争性阻滞剂CGRP8-37阻断。CGRP(10-10～3×10-8mol/L)对PE预收缩的内皮完整血管和去内皮血管产生浓度依赖性的舒张作用,其中内皮完整组的血管舒张作用比去内皮组更明显,两者相比CGRP在3×10-9～3×10-8mol/L时,差异有统计学意义(P<0.05)。P物质对PE预收缩的内皮完整血管无舒张作用。结论:CAP对大鼠肠系膜阻力动脉具有部分内皮依赖性舒张作用,该作用机制与其促进内皮一氧化氮合酶(NOS)途径有关;CAP的非内皮依赖性舒张血管效应与CGRP释放有关。Objective： To investigate the vasodilating effect of eapsaicin （CAP） on rat mesenterie artery and its mechanism. Methods： The third branch of the superior mesenteric artery in male Sprague- Dawley rat（250- 350 g） was excised,the periadventitial fat and connective tissue were removed and the mesenteric artery was dissected into 2 mm rings. Each ring was placed in a 5 ml organ bath of DMT 610M system and the tension was recorded. Results： CAP（ 10-9 - 10-5 tool/L） relaxed endothelium-intact and endothelium-denuded mesenterie artery pre-constricted by phenylephrine （10-5 mol/L）, and the vasodilation in endothelium-intact mesenteric artery was stronger than that in endothelium-denuded one.Pretreatment with either L-NAME （ 3 x 10 -4 mol/L）, an inhibitor of nitric oxide synthase （ NOS ）, or CGRP8-37（2 x 10-6 tool/L）, an antagonist of ealcitonin gene-related peptide （CGRP）, for 30 min significantly attenuated the relaxation of endothelium-intact mesenteric artery induced by CAP. CGRP （10-l~ -3 x 10-s mol/L） relaxed endothelium-intact and endothelium-denuded mesenteric artery pre- constricted by phenylephrine, and the vasodilation in endothelium-intact mesenteric artery was stronger than that in endothelium-denuded one. Substance P did not relax the mesenteric artery pre-constricted by phenylephrine. Conclusions： CAP has partial endothelium-dependent relaxation effect on rat mesenteric artery, which may be mediated by activating the endothelial NOS-NO pathway. The endothelium- independent relaxation in rat mesenteric artery induced by CAP may be mediated by CGRP.

关 键 词：辣椒素/药理学 肠系膜动脉/药物作用 血管舒张 降钙素基因相关肽 内皮 

分 类 号：R285.5[医药卫生—中药学]