一株脂肽产生菌发酵参数的响应曲面法优化  

Parameter Optimization by Response Surface Methodology for Lipopeptide Production of Bacillus sp. BO2

作　　者：单大鹏[1,2,3] 李旭[1,2,3] 产竹华[1,2,3] 刘洋[1,2,3] 邵宗泽[1,2,3] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]国家海洋局海洋生物遗传资源重点实验室,福建厦门361005 [3]福建省海洋生物技术重点实验室,福建厦门361005

出　　处：《海洋科学进展》2012年第4期575-586,共12页Advances in Marine Science

基　　金：国家海洋局第三海洋研究所基本科研业务费项目--产芽孢石油降解菌的分离与鉴定(海三科2009042);国家海洋局青年海洋科学基金项目--海洋微生物表面活性物质发酵制备工艺研究(2011142);福建省自然科学基金项目--海岸带石油降解微生物资源的初步研究(2010J01206)

摘　　要：从渤海污染土壤中分离的表面活性剂产生菌,经鉴定为Bacillus sp.BO2,该菌在对数生长期产生表面活性剂,至稳定期后可将发酵液的表面张力降低至31.0mN.m-1。初步分析发现其表面活性剂成分为环脂肽,该脂肽在不同温度、盐度、pH条件下有较好的稳定性。进一步采用响应曲面法(RSM),以乳化系数作为响应值来优化菌株BO2产生物表面活性剂培养条件。还通过实验确定碳源(液体石蜡)、NH4NO3、KH2PO4为3个影响表面活性剂产量的显著因素,再进行发酵培养基优化,获得最佳培养基配方。获得的发酵参数为利用该菌株发酵生产生物表面活性剂提供数据,为石油降解菌的应用开发提供了有价值的信息。A lipopeptide-producing bacterium Bacillus sp. BO2 was isolated from coastal soils of the Bohai Sea. It can produce a cyclic lipopeptide that can lower surface tension of culture liquid to 31.0 mN m This lipopeptide was stable in wide ranges of temperature, salinity and pH. Furthermore, Box-Behnken design was used to optimize fermentation condition of the Bacillus to produce lipopeptide surfaetant. I.iq uid paraffin, NH4N（）3 and KH2P（）4 were selected to be the three key factors affecting yield of surfactant by the Plackett-Burman experiment. Based on this, with the response surface method in the Box-Behnken design optimization, the fermentation parameters including exact medium composition were optimized theo- retically, and verified experimentally. The optimized parameters will contribute to the application of BO2 as a biosurfactant producer.

关 键 词：生物表面活性剂 芽孢杆菌 响应曲面法(RSM) Plackett—Burman设计 BOX-BEHNKEN设计 

分 类 号：Q939.9[生物学—微生物学]