水飞蓟素对载脂蛋白E基因敲除小鼠动脉粥样硬化的影响  

Anti-atherosclerosis effect and its mechanisms ofsilymarin in ApoE-deficient mice

作　　者：马翔宇[1] 张金盈[1] 张辉[1] 张力[1] 沈德良[1] 杨威[1] 

机构地区：[1]郑州大学第一附属医院心内科,郑州450052

出　　处：《临床心血管病杂志》2013年第4期290-292,共3页Journal of Clinical Cardiology

摘　　要：目的:观察水飞蓟素对载脂蛋白E(ApoE)基因敲除(ApoE-/-)小鼠动脉粥样硬化的影响,并探讨其可能机制。方法:高脂喂养加缩窄性颈动脉套管术诱导小鼠动脉粥样硬化形成。实验分对照组(A组)、水飞蓟素低剂量组(B组)与水飞蓟素高剂量组(C组)。给药4周后取颈动脉标本,测量斑块面积、纤维帽厚度及脂质含量;检测血脂及血清丙二醛、超氧化物歧化酶水平。结果:①A、B两组间血脂水平差异无统计学意义,C组较A、B两组血脂均降低(P<0.05);②与A组相比,B、C两组斑块面积及脂质含量均减少(P<0.05),纤维帽厚度均增加(P<0.05),且C组较B组改变更显著(P<0.05);③与A组相比,B、C两组血清丙二醛水平均降低(P<0.05),超氧化物歧化酶水平均增高(P<0.05),且C组较B组改变更显著(P<0.05)。结论:水飞蓟素对ApoE-/-小鼠已形成的动脉粥样硬化斑块进展有明显抑制作用,其机制可能与降脂和抗氧化应激有关。Objective：To examine the effect of silymarin on atherosclerosis in apolipoprotein E-deficient（ApoE-/-） mice,and explore the potential mechanisms. Method：Carotid atherosclerotic lesions were induced by high-fat diet and perivascular constrictive collars placement.ApoE-/-mice were randomly allocated into three groups： control group（group A）,low-dose silymarin group（group B） and high-dose silymarin group（group C）.Four weeks after drug administration,common carotid arteries were removed to determine plaque area,cap thickness and lipid.Serum lipid,malondialdehyde and superoxide dismutase were measured as well. Result：①There were no significant differences in serum lipid between group A and B.Compared with other groups,group C had a lower level of serum lipid（P0.05）.②Compared with group A,plaque area and lipid were decreased in group B and C（P0.05） while cap thickness increased（P0.05）.Furthermore the changes of group C were more significant（P0.05）.③Serum malondialdehyde was lower in group B and C（P0.05） than that in group A,in the meanwhile superoxide dismutase was higher（P0.05）.In addition the changes of group C were more significant（P0.05）. Conclusion：Silymarin can evidently inhibit the progress of existing atherosclerotic lesions in ApoE-/-mice.The possible mechanisms are lowering serum lipid levels and inhibiting oxidative stress.

关 键 词：动脉粥样硬化 水飞蓟素 APOE基因敲除小鼠 丙二醛 超氧化物歧化酶 

分 类 号：R543.5[医药卫生—心血管疾病]