嗜热四膜虫Ran结合蛋白1的表达对大小核分裂的影响  

Ran Binding Protein 1 Affects Macronuclear and Micronuclear Division in Tetrahymena thermophila

作　　者：赵丹[1] 梁海霞[1] 许静[1] 王伟[1] 

机构地区：[1]山西大学生物技术研究所,化学生物学与分子工程教育部重点实验室,太原030006

出　　处：《中国生物化学与分子生物学报》2013年第4期330-337,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30770295;No.31072000);教育部科学技术研究重点项目(No.201026)资助~~

摘　　要：Ran是细胞内的一种具有GTP酶活性的功能蛋白,可以调节染色体稳定性、细胞核组建以及核质运输等多种细胞进程.Ran结合蛋白1(Ran-binding protein 1,Rbp1p)是Ran的必要调控因子,促进Ran-GTP水解为Ran-GDP.本研究从嗜热四膜虫大核基因组中鉴定出1个保守的Ran结合蛋白基因RBP1(TTHERM_00158040,http://www.ciliate.org).实时荧光定量PCR表明,RBP1在四膜虫营养生长和有性生殖过程中都有表达,且在有性生殖过程中表达水平提高.免疫荧光定位表明,在营养生长期Rbp1p定位于细胞质中.过表达RBP1或敲减RBP1后,细胞生长速率下降,大核的无丝分裂异常,细胞分裂末期产生了无大核的异常细胞,同时过表达RBP1导致了多小核的产生.结果表明,Rbp1p影响四膜虫细胞核的分裂进程,它的正常表达对细胞增殖过程起到重要的调节作用.Ran regulates a variety of cellular processes via its intrinsic GTPase activity including chromosome stability, formation of the nucleus and nucleocytoplasmic transport. Ran-binding protein 1 （Rbplp） facilitates the activation of Ran GTPase as a necessary regulator in the Ran GTPase cycle. We identified an evolutionary conservative Ran-binding protein 1 gene from Tetrahymena thermophila termed RBP1 （TTHERM 00158040, http：//www, ciliate, org） which expressed in growing, starvation and conjugation stage. HA-tagged Rbplp was localized in the cytoplasm during vegetative growing stage. Over-expression or suppressed expression of Rbplp caused abnormal macronuclear amitosis or multi- micronuclear cells. These results that Rbplp affects of its normal expression in Tetrahymena thermophila the nuclear division suggested the important function

关 键 词：Ran结合蛋白1 嗜热四膜虫 核分裂 

分 类 号：Q952[生物学—动物学]