HSF4B通过上调RhoA和Rac1促进晶状体上皮细胞迁移  

HSF4B Increases Migration of Lens Epithelial Cell by Up-regulating the Expression of RhoA and Rac1

作　　者：张军[1] 马增翼[2] 李淑莲[1] 刘广超[1] 胡延忠[1] 

机构地区：[1]河南大学医学院细胞与分子免疫学重点实验室,河南开封475000 [2]复旦大学附属华山医院神经外科,上海200040

出　　处：《中国生物化学与分子生物学报》2013年第4期377-382,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助(No.30871299;No.81270985)~~

摘　　要：热休克转录因子4(heat shock transcription factor 4,HSF4)是调控新生儿期晶状体发育的关键转录因子.它参与调控晶状体上皮细胞增生和纤维细胞分化,然而其作用机理仍不清楚.利用鼠晶状体上皮细胞mLEC/HSF4-/-和mLEC/HA-HSF4b细胞为材料,对HSF4在晶状体上皮细胞中的调控作用进行研究.结果发现,重建的mLEC/HA-HSF4b细胞中,高表达热休克蛋白25(Hsp25)和αB晶体蛋白(αB-crystallin).细胞划痕实验和Transwell细胞迁移实验结果表明,mLEC/HSF4-/-细胞明显比mLEC/HA-HSF4b细胞的迁徙速度慢(t检验,P=0.0001).应用免疫印迹和半定量RT-PCR实验发现,HSF4b能上调RhoA,Rac1和CDC42的表达.但后者的表达不受Hsp25和αB-crystallin调控.由此推论,HSF4b通过调控RhoA,Rac1和CDC42的表达参与调控晶状体上皮细胞向后房的迁徙.这一新发现对解释HSF4b调控晶状体发育提供了有力的证据.Heat shock transcription factor 4 （HSF4） is a key regulator of postnatal lens development by involving the regulation of lens epithelial cell proliferation and fibroblast differentiation. We found that the expression of Hsp25 and aB-crystallin was up-regulated in HSF4b-reconstituted mLEC/HA-HSF4b cells. The results of wound-healing and Transwel! assays indicated that the migration of mLEC/HA-HSFdb cells was significantly faster than that of mLEC/HSF4-/-cells （ P = 0. 0001 ）. The immunoblotting and semi- quantity RT-PCR data showed that HSF4b up-regulated the expression of RhoA, Racl and CDC42 and correlated with the Hsp25 and ctB-crystallin levels, except for CDC42. We hypothesized that HSF4b might regulate the migration of anterior epithelial cell to posterior by increasing the expression of RhoA and Racl.

关 键 词：热休克转录因子4 晶状体上皮细胞 RHOA RAC1 热休克蛋白25 αB-晶体蛋白 

分 类 号：Q78[生物学—分子生物学]