大鼠急性肺损伤病理变化的形态计量研究  

Morphometrical study of pathological changes in rats with acute lung injury

作　　者：王海燕[1] 赵玉金[1] 杜娟[1] 李海胜[1] 杨策[1] 詹俊[1] 岳彩黎[1] 蒋建新[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所第四研究室,创伤、烧伤与复合伤国家重点实验室,重庆400042

出　　处：《中华创伤杂志》2013年第4期364-367,共4页Chinese Journal of Trauma

基　　金：国家重点基础研究发展计划资助项目（2012CB518104）;创伤、烧伤与复合伤国家重点实验室自主研究课题资助项目（SKLZZ201104）

摘　　要：目的用体视学方法定量研究大鼠经胸部撞击复合内毒素所致急性肺损伤（acutelunginjury，ALI）模型的组织病理学特点及变化规律。方法12只sD大鼠按随机数字表法分为正常对照组、ALI致伤1，2，7d组，每组3只。各组取肺组织行HE染色，分别测试肺实质百分比、肺泡间隔厚度、肺泡截面积及肺泡腔内炎性细胞计数。结果Au致伤1，2d组肺泡腔内炎性细胞计数分别为（13．08±6．60）个、（26．81±16．86）个，正常对照组为（0．89±0．78）个，致伤组均较对照组明显增加（P〈0．01）。Au致伤1，2，7d组肺实质百分比分别为（23．77±3．91）％、（27．32±5．95）％、（44．57±6．46）％，正常对照组为（19．44±2．48）％，致伤组均较对照组明显增加（P〈0．01）。Au致伤1，2，7d组肺泡间隔分别为（3．50±2．18）μm、（7．67±7．14）μm、（17．23±13．08）μm，正常对照组为（2．87±1．75）μm，致伤组均较对照组逐渐增厚（P〈0．05）。ALI致伤1，2，7d组肺泡截面积分别为（1653．90±950．77）μm2、（1328．15±802．30）μm2、（1055．20±1002．75）μm2，正常对照组为（1641．30±906．60）μm2，致伤组均较对照组逐渐减少（P〈0．05）。结论大鼠经胸部撞击后复合静脉注射内毒素可建立病理形态上符合Au的动物模型。通过体视学方法定量检测可反映ALI的组织病理学特点及变化规律。Objective To quantitatively study the histopathological features and its variation rules in rat models of acute lung injury （ALI） induced by chest impact accompanied with endotoxin infection by stereological method. Methods Twelve SD rats were randomly divided into control group and groups at 1,2, and 7 days after ALl, with 3 rats per group. HE staining of lung tissue specimens was performed for each group so as to detect percentage of lung parenchyma, thickness of alveolar septum, alveolar section area and count of inflammatory cells in alveolar space. Results Inflammatory cells in alveolar space in groups at 1 and 2 days after ALI and control group counted 13.08 -＋ 6.60, 26.81 -＋ 16.86 and 0. 89 ＋ 0.78 respectively, with a far higher number in the injured groups （ P 〈 0.01 ）. Percentage of lung parenchyma in groups at 1,2, and 7 days after ALI and control group was （23.77 ＋ 3.91 ） % , （ 27.32 ＋ 5.95 ） % , （44.57 ＋ 6.46） % and （ 19.44 ＋ 2.48） % respectively, with a far higher proportion in the injured groups （P 〈 0.01）. Alveolar septum in the groups at 1 and 2 days after ALI and control group was （3.50 ＋ 2.18 ） μm, （7.67 ＋ 7.14） μm, （ 17.23 ＋ 13.08 ） μm, and （ 2.87 ＋ 1.75 ） μm respectively, with a gradual thickening in the injured groups （ P 〈 0.05 ）. Alveolar section area in groups at 1, 2, and 7 days after ALI and control group was （1 653.90 ＋950.77） μm2, （ 1 328.15 ＋ 802.30） μm2, （ 1 055.20 ＋ 1 002.75 ） μm2, and （ 1 641.30 ＋ 906.60） μm2 respectively,with a gradual decline in the injured groups （P 〈 0.05）. Conclusions Chest impact with intravenous administration of endotoxin on rats sets up models that accord with ALI in pathomorphology successfully. Quantitative study by stereological method reflects features and variation rules of histopathology of ALI.

关 键 词：急性肺损伤 模型 动物 形态计量 

分 类 号：R563.8[医药卫生—呼吸系统]