五个WRKY转录因子在水稻叶片生长和抗病反应中的表达研究  

Characteristic Expression Analysis of Five WRKY Transcriptional Factors in Rice Leaf Growth and Disease Resistance Reaction

作　　者：徐文静[1] 缪刘杨[1] 李莉云[1] 刘钊[1] 刘雨萌[1] 江光怀[2] 杨凤环[3] 何晨阳[3] 刘国振[1] 

机构地区：[1]河北农业大学生命科学学院,保定071000 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]中国农业科学院植物保护研究所,北京100094

出　　处：《生物化学与生物物理进展》2013年第4期356-364,共9页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(31171528);supported by a grant from The National Natural Science Foundation of China(31171528)

摘　　要：WRKY是植物基因组中最大的转录因子家族之一,它们在抗病及其他信号转导途径中发挥着重要的调控作用.为了解水稻WRKY的功能,我们选择了5个WRKY转录因子,用免疫印迹技术调查了它们在水稻叶片生长和在Xa21介导的白叶枯病抗性反应中的表达丰度变化.结果表明,OsWRKY13、23和71在叶片中表达,且随叶片生长而逐步增加,至成熟期略有下降,但在叶片中检测不到OsWRKY45和OsWRKY53的表达信号.在Xa21介导的白叶枯病抗性反应中,OsWRKY45、53和71均受诱导表达,而OsWRKY13和OsWRKY23蛋白质的表达没有可见的变化.进一步比较OsWRKY45、OsWRKY53和OsWRKY71在抗、感和对照(Mock)反应中的表达,发现它们在抗、感反应中均发生相似变化.上述结果说明,OsWRKY13和OsWRKY23可能在叶片正常生长过程中发挥作用,OsWRKY45和OsWRKY53可能在水稻-白叶枯病菌互作过程中发挥作用,而OsWRKY71在二种条件下均有功能.WRKY transcription factors,one of the largest gene families in rice genome,are reported to play important roles during disease resistance and other signaling pathways.We chose five WRKY proteins to survey their expression profiles in the process of leaf growth and the Xa21-mediated Xanthomonas oryzae pv.oryzae（Xoo） resistance responses by Western blotting.The results indicated that OsWRKY13,OsWRKY23 and OsWRKY71 were expressed increasingly with leaf growth till the flowering stage and their expression were declined slightly at filling stage.However,the expression of OsWRKY45 and OsWRKY53 were not detected.In the Xa21-mediated resistance response,OsWRKY45,OsWRKY53 and OsWRKY71 proteins were induced,while the expression of OsWRKY13 and OsWRKY23 proteins were stable.Comparison among the resistance reaction,susceptible reaction and mock treatment revealed that the expression of OsWRKY45,OsWRKY53 and OsWRKY71 proteins had a similar pattern between resistance and susceptible reactions.This result suggested that OsWRKY13 and OsWRKY23 may play roles in rice leaf growth,OsWRKY45 and OsWRKY53 may play roles in rice-Xoo interactions and OsWRKY71 is functioning in both situations.

关 键 词：WRKY 水稻 XA21 白叶枯病菌 转录因子 

分 类 号：Q945.8[农业科学—植物病理学] Q7[生物学—植物学]