NOD1与NOD2在变应性鼻炎中的表达及糖皮质激素对其的调节  

The expression of NOD1 and NOD2 and the regulation of glucocorticoids on them in allergic rhinitis

作　　者：胡双[1] 游学俊[1] 曹平平[1] 刘争[1] 崔永华[1] 

机构地区：[1]华中科技大学同济医院耳鼻咽喉-头颈外科,武汉430030

出　　处：《临床耳鼻咽喉头颈外科杂志》2013年第8期393-396,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金(No:81020108018)

摘　　要：目的:探讨NOD1与NOD2在变应性鼻炎(AR)患者鼻黏膜中的表达及糖皮质激素对其的调控作用。方法:收集13例AR患者下鼻甲黏膜及19例单纯鼻中隔偏曲患者下鼻甲黏膜(对照组),采用免疫组织化学方法和实时定量RT-PCR方法检测下鼻甲黏膜样本中NOD1、NOD2的表达。另外收集5例单纯鼻中隔偏曲患者下鼻甲黏膜及5例AR患者下鼻甲黏膜,采用体外组织块培养方法,观察糖皮质激素对NOD1和NOD2表达的调节作用。结果:NOD1和NOD2mRNA及蛋白在AR组中的表达较对照组明显增高(P<0.05),免疫组织化学显示NOD1与NOD2在上皮层和黏膜固有层都有表达。组织培养结果表明,用糖皮质激素刺激后,AR患者鼻黏膜中NOD1和NOD2的表达显著下降(P<0.05)。结论:NOD1和NOD2作为先天性模式识别受体可能参与了AR的发病过程;糖皮质激素可能通过抑制NOD1和NOD2的表达而达到治疗AR的作用。Objective：To investigate the expression of NOD1 and NOD2 in the nasal mucosa of healthy individuals and allergic rhinitis（AR）,and explored the regulation of glucocorticoids on them.Method：RT-PCR and immunohistochemistry were used to detect the expression of NOD1 and NOD2 in nasal mucosa from healthy control and AR.Nasal explant culture was used to explore the effect of glucocorticoids on NOD1 and NOD2 expression.Result：NOD1 and NOD2 mRNA expression level was significantly increased in AR compared with control.Immunohistochemical staining demonstrated that NOD1 and NOD2 were mainly expressed by epithelial cells and some unknown cells in lamina propria and there were significantly more positive staining cells were observed in AR tissue when compared with control.Glucocorticoids down-regulated NOD1 and NOD2 expression in AR.Conclusion：NOD1 and NOD2 as two PRRs may take part in the pathogenesis of AR,glucocorticoids may play a therapeutical role on allergic rhinitis through down-regulated the expression of NOD1 and NOD2.

关 键 词：鼻炎 变应性 NOD1 NOD2 糖皮质激素类 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]