电针对脑心综合征大鼠心肌组织1-磷脂酰肌醇3-激酶、缺氧诱导因子-1α及血管表皮生长因子表达的影响  

Effect of Electroacupuncture on Expression of Myocardial PI 3 K,HIF-1α and VEGF in Rats with Cerebral-cardiac Syndrome

作　　者：吴生兵[1] 曹健[1] 高纺[1] 王丽娜[1] 常梦娟[1] 薛晶晶[1] 张田宁[1] 周美启[1] 

机构地区：[1]安徽中医学院,省部共建新安医学教育部重点实验室,合肥230038

出　　处：《针刺研究》2013年第2期87-92,91-92,共6页Acupuncture Research

基　　金：安徽省自然科学基金资助项目(No.11040606M188;No.1208085QH 160);安徽高校省级科学研究项目(No.KJ 2013Z176)

摘　　要：目的:观察电针对脑心综合征(CCS)大鼠组织1-磷脂酰肌醇3-激酶(PI 3K)、缺氧诱导因子-1α(HIF-1α)及血管表皮生长因子(VEGF)表达的影响,探讨电针对CCS大鼠心肌组织损伤保护的作用机制。方法:从70只SD大鼠中随机选择10只作为假手术组,其余60只用于CCS模型复制,采用胶原酶加肝素联合注射于尾状核复制CCS大鼠模型。选取造模成功的大鼠30只,分为模型组、电针组和非经非穴组,每组10只。采用胶原酶加肝素联合注射于大鼠尾状核的方法造模。造模第1天开始电针,电针组选取"水沟""风府""内关"和"心俞"穴,非经非穴组选取臀部非经非穴点4处,每次20min,每天1次,连续3次。采用免疫组化法分别检测心肌组织PI 3K、HIF-1α及血管表皮生长因子VEGF表达。结果:与假手术组比较,模型组心肌组织PI 3K、HIF-1α及VEGF大量表达(P<0.01);与模型组比较,非经非穴组心肌组织PI 3K、HIF-1α及VEGF的变化差异无统计学意义(P>0.05),而电针组心肌组织PI 3K、HIF-1α及VEGF大量增加(P<0.05)。结论:电针干预CCS可能通过激活PI 3K后上调HIF-1α的表达,从而激活VEGF,起到保护CCS受损心肌的作用。Objective To observe the effect of electroacupuncture （EA） on the expression of myocardial 1-phosphati- dylinositol 3-kinase （PI 3 K）, hypoxia-inducible factor-1α （HIF-1α） and vascular endothelial growth factor （VEGF） in rats with ce- rebral-cardiac syndrome （CCS）, so as to reveal its mechanism underlying reducing ischemic myocardial injury. Methods Forty SD rats were randomly and equally divided into sham-operation, model, EA and non-acupoint （the lateral-superior side of the hip） groups （10 rats/group）. CCS model was established by injection of collagenase （1 U/μL） and heparin （7 U/μL） into the right cau- date nucleus. Following modeling, EA （1.5 mA, 2 Hz, 20 rain） was applied to ＂Shuigou＂ （GV 26）, ＂Fengfu＂ （GV 16）, ＂Neiguan＂ （PC 6） and ＂Xinshu＂ （BL 15） acupoints, once daily for three consecutive days. The expression levels of PI 3 K,HIF-la and VEGF in the myocardium were detected by immunohistochemistry. Results Compared with the sham-operation group, the expression levels of myocardial PI 3 K, HIF-la and VEGF proteins were significantly increased in the model group （P〈0.01）. While in comparison with the model group, there were little increase in the non-acupoint group （P〉0.05） and considerable in- crease in the expression levels of the 3 myocardial proteins in the EA group （P〈0.05）. Conclusion EA intervention has a function in upregulating the expression of myocardial VEGF, HIF-1α and PI 3 K proteins in CCS rats, which maybe contribute to its protective effect on ischaemic myocardial injury.

关 键 词：电针脑心综合征 心肌 1-磷脂酰肌醇3-激酶 缺氧诱导因子-1Α 血管表皮生长因子 血管生成 

分 类 号：R245.97[医药卫生—针灸推拿学]