电针“丰隆”穴对高脂血症大鼠血脂、肝脏组织中ATP结合盒转运子A1 mRNA和蛋白表达的影响  

Effects of Electroacupuncture Stimulation of "Fenglong"(ST 40) on Expression of Liver ATP-binding Cassette Transporter A1 mRNA and Protein in Rats with Hyperlipidemia

作　　者：张红星[1] 武欢[1] 黄浩[1] 肖颖[1] 王东[1] 

机构地区：[1]武汉市中西医结合医院针灸科,武汉430022

出　　处：《针刺研究》2013年第2期100-105,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.30873309)

摘　　要：目的:探讨电针"丰隆"穴对高脂血症大鼠血脂的影响及作用机制。方法:40只健康SD大鼠随机分为空白组、模型组、饮食控制组、电针组及电针+饮食控制组,每组8只。空白组喂普通饲料,其余4组采用高脂饮食饲料饲喂法建立高脂血症大鼠模型,造模28d后,饮食控制组、电针+饮食控制组改用普通饲料喂养,其余3组饲喂方法不变,同时电针组及电针+饮食控制组电针"丰隆"穴,疏密波,频率2Hz/100Hz,强度2mA治疗30min,每日1次,治疗28d。治疗结束后,检测各组大鼠血浆总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量,并用原位杂交法、实时定量聚合酶链反应法及免疫印迹法检测各组大鼠肝脏组织中ATP结合盒转运子A 1(ABCA 1)mRNA和蛋白水平。结果:模型组大鼠TC、LDL-C水平较空白组显著上升(P<0.01),肝脏ABCA 1mRNA和蛋白含量均显著降低(P<0.01);与模型组比较,电针组、电针+饮食控制组大鼠TC、LDL-C水平显著降低,肝脏ABCA 1mRNA和蛋白含量均显著升高(P<0.01);与饮食控制组比较,电针+饮食控制组大鼠TC、LDL-C水平显著降低,肝脏ABCA 1的mRNA和蛋白含量均显著升高(P<0.01)。结论:电针"丰隆"穴可通过上调高脂血症大鼠肝组织ABCA 1蛋白及mRNA表达,促进由其介导的胆固醇逆转运,降低胆固醇负荷而防治高脂血症。Objective To observe the effect of electroacupuncture （EA） at ＂Fenglong＂ （ST 40） on blood lipid levels and hepatic ATP binding cassette transporter A 1 （ABCA 1 ） mRNA and protein expression in hyperlipidemia rats, so as to study its mechanism underlying improvement of HLP. Methods Forty SD rats were randomized into normal control, model,diet control, EA, and EA＋ diet control groups, with 8 rats in each group. Hyperlipidemia model was established by feeding the animals with high fat forage for 28 days. After modeling, rats of the two diet control groups were fed with basal forage. EA （2 mA, 2 Hz/ 100 Hz） was applied to bilateral ST 40 for 30 rain, once daily for 28 days. Plasma total cholesterol （TO）, triglyceride （TG）, low- density lipoprotein cholesterol （LDL-G）, and high-density lipoprotein cholesterol （HDL-C） contents were detected by automatic biochemistry analyzer, and the expression levels of ABCA 1 mRNA and protein in the liver tissue were assayed by in situ hybri- dization（ISH）, RT-PCR and Western blot, respectively. Results Compared with the normal control group, the contents of plas- ma TC and LDL-C were apparently increased （P〈0.01） and the expression levels of hepatic ABCA 1 mRNA （detected by both ISH and RT-PCR） and ABCA 1 protein were significantly decreased in the model group （P〈0.01）. In comparison with the model group, plasma TC and LDL-C contents were significantly decreased and the expression levels of hepatic ABCA 1 mRNA and pro- tein were significantly up-regulated in the EA group and EA＋ diet control group（ P〈0. 01 ）. The effects of EA ＋ diet control were significantly superior to those of diet control in down-regulating plasma TC and LDL-C levels and up-regulating hepatic ABCA 1 mR- NA and protein expression levels（P〈0.01）. Cenclesion EA stimulation of ＂Fenglong＂（ST 40） can decrease the load of bloodcholesterol and suppress the down-regulation of hepatic ABCA 1 mRNA and protein expression in hyperlipidemia rat

关 键 词：高脂血症 电针ATP结合盒转运子A 1 基因和蛋白表达 

分 类 号：R245.97[医药卫生—针灸推拿学]