不同电针参数组合针刺助产处方对孕晚期大鼠内分泌激素的影响  

Influence of Electroacupuncture Stimulation of Delivery-facilitating Acupoints with Different Stimulating Parameters on Serum Endocrine Hormones of Late-stage Pregnant Rats

作　　者：苑鸿雯[1] 陈滢如[1] 舒福政[1] 李春华[1,2] 刘玉祁[1,3] 马良宵[1,4] 任晓暄[1] 张鹏[1] 李静[1] 薛立文[1,5] 辛思源[1] 朱江[1,4] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]首都医科大学电力教学医院,北京100073 [3]中国中医科学院中医临床基础医学研究所,北京100700 [4]国家中医药管理局针灸特色疗法评价重点研究室,北京100029 [5]首都医科大学附属北京中医医院针灸科,北京100010

出　　处：《针刺研究》2013年第2期112-117,共6页Acupuncture Research

基　　金：国家自然科学基金面上资助项目(No.30973793);国家重点基础研究发展计划("973"计划)资助项目(No.2012CB 518506);教育部博士点基金资助项目(No.20090013110005)

摘　　要：目的:通过观察不同电针参数组合针刺对晚孕大鼠体内相关内分泌激素的影响,探讨不同电针参数组合助产处方促分娩效应的内分泌机制。方法:健康成年雌性Wistar大鼠96只,随机分为7个妊娠组与1个正常对照组,妊娠组大鼠采用观察阴栓法制备妊娠模型。受孕后随机分为15Hz组、30Hz组、50Hz组、2Hz/15Hz组、2Hz/30Hz组、2Hz/50Hz组和1个妊娠对照组。电针组均在电针双侧"合谷"穴20min后加针双侧"三阴交"穴5min,强度0.2～0.3mA,根据组别选取不同电针波形及频率。应用酶联免疫吸附测定法检测血清雌二醇(E2)、孕酮(P)和前列腺素E2(PGE2),并计算雌孕激素比(E2/P)。结果:与正常对照组比较,妊娠对照组血清E2、P和PGE2含量均显著增高(P<0.01)。与妊娠对照组比较,15Hz组、30Hz组、50Hz组及2Hz/15Hz组、2Hz/50Hz组血清E2含量均显著增高(P<0.01,P<0.05);15Hz组、30Hz组、50Hz组以及2Hz/50Hz组血清P含量均显著下降(P<0.01,P<0.05),且E2/P比值均显著增高(P<0.01);15Hz组、30Hz组及2Hz/50Hz组血清PGE2含量均显著增高(P<0.05)。2Hz/50Hz组血清E2和PGE2含量高于其它电针组。结论:电针助产处方可通过调节孕鼠血清E2、P和PGE2的含量,以及促进E2、P的失衡,促进子宫收缩,从而达到促分娩的目的。但不同电针参数的调节效应有一定差异。2Hz/50Hz疏密波促进宫缩的效应更为明显,为针刺助产处方的推荐电针参数组合。Objective To observe the influence of electroacupuncture （EA） stimulation of ＂Hegu＂ （LI 4） and ＂Sanyin- jiao＂（SP 6; a classical delivery-facilitating acupoint recipe） at different parameters on endocrine hormone levels of late-stage pregnant rats, so as to find the better stimulation parameters for delivery facilitation. Methods Ninety-six female Wistar rats were randomly divided into eight groups： normal control （normal）, pregnancy （model）, EA-15 Hz, EA-30 Hz, EA-50 Hz, EA-2 Hz/ 15 Hz, EA-2 Hz/30 Hz and EA-2 Hz/50 Hz, with 12 rats in each group except EA-30 Hz group （n = 10）. EA stimulation （0.2 to 0.3 mA） was delivered to bilateral LI 4 for 20 min and then bilateral SP 6 for 5 min （25 min）. The contents of serum estradiol（E2）, progesterone （P） and prostaglandin E2 （PGE2） were detected by ELISA. Results Compared with the normal group, se- rum E2, P and PGE2 contents were significantly increased in the pregnancy model group （P〈0.01）, and E2/ P level was down- regulated mildly （P〉0.05）. Compared with the model group, the contents of serum E2 in the EA-15 Hz, EA-30 Hz, EA-50 Hz, EA-2 Hz/15 Hz and EA-2 Hz/50 Hz groups, serum PGE2 contents in the EA-15 Hz, EA-30 Hz and EA-2 Hz/50 Hz groups, and E2/P levels in the EA-15 Hz, EA-30 Hz, EA-50 Hz and EA-2 Hz/50 Hz groups were significantly increased （ P〈0.01, P〈0.05）, whereas serum P contents in the EA-15 Hz, EA-30 Hz, EA-50 Hz and EA-2 Hz/50 Hz groups were significantly down-regulated （P〈0.01, P〈0.05）. The effects of EA-2 Hz/50 Hz group were significantly superior to those of EA-2 Hz/15 Hz and EA-2 Hz/ 30 Hz groups in raising serum E2 and E2/P levels （P〈0.05, P〈0.01）. No significant differences were found among the EA- 15 Hz, EA-30 Hz, EA-50 Hz and EA-2 Hz/50 Hz groups in raising serum E2, PGE2 and E2/P levels, and among the EA-30 Hz, EA- 50 Hz and EA-2 Hz/50 Hz groups in lowering serum P levels （ P〉0.05）. It displayed that the effects of EA at 15 Hz, 30 Hz and 2 Hz/50 Hz on t

关 键 词：促分娩 电针 合谷穴 三阴交穴 刺激频率 雌二醇 孕酮 前列腺素E2 

分 类 号：R245.97[医药卫生—针灸推拿学]